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PYK2 (Phospho-Tyr580) Antibody

Catalog No : A0084
  • Applications
    WB, IHC, ELISA
  • Reactivity
    Human, Mouse, Rat
  • Size
    50ul
  • Price
    $195.00
Anti-PYK2 (Phospho-Tyr580) polyclonal Antibody detects PYK2 only when phosphorylated at Tyr580. Validated for WB, IHC and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
  • Product Name
    PYK2 (Phospho-Tyr580) Antibody
  • Catalog No
    A0084
  • Storage/Stability
    Stable at -20°C for at least 1 year.
  • Applications
    WB, IHC, ELISA
  • Clonality
    Polyclonal
  • Host Species
    Rabbit
  • Reactivity
    Human, Mouse, Rat
  • Application Dilutions
    WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000
  • Database Links
    Gene ID: 2185, Swiss-Prot #: Q14289, OMIM #: 601212, Unigene #: Hs.491322
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human PYK2 around the phosphorylation site of Tyr580.
  • Immunogen Range
    546-595
  • Modification Sites
    H: Y580 M: Y580 R: Y580
  • MW
    115 kDa
  • NCBI Gene Symbol
    PTK2B
  • Physical Form
    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Purification
    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
  • Specificity
    PYK2 (Phospho-Tyr580) Antibody detects endogenous levels of PYK2 only when phosphorylated at Tyr580.
  • Synonyms
    CADTK, CAK beta, Calcium-dependent tyrosine kinase, Cell adhesion kinase beta, FADK 2, FAK2, Focal adhesion kinase 2, kinase Pyk2, Proline-rich tyrosine kinase 2, Protein tyrosine kinase 2 beta, PTK2B, RAFTK, Related adhesion focal tyrosine kinase
  • Target Modification
    Phospho
Application Images
Western blot analysis of lysates from K562 cells, using PYK2 (Phospho-Tyr580) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human brain, using PYK2 (Phospho-Tyr580) Antibody. The picture on the right is blocked with the phospho peptide.
Western Blot analysis of K562 cells using Phospho-PYK2 (Y580) Antibody
Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100(4°,ON). Negative control (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Western blot analysis of lysates from K562 cells, using PYK2 (Phospho-Tyr580) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human brain, using PYK2 (Phospho-Tyr580) Antibody. The picture on the right is blocked with the phospho peptide.
Western Blot analysis of K562 cells using Phospho-PYK2 (Y580) Antibody
Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100(4°,ON). Negative control (right) obtaned from antibody was pre-absorbed by immunogen peptide.