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POLR2A (Phospho-Ser1619) Antibody

Catalog No : A0082
  • Applications
    WB, IHC, IF, ELISA
  • Reactivity
    Human, Mouse, Rat
  • Size
    50ul
  • Price
    $195.00
Anti-POLR2A (Phospho-Ser1619) polyclonal Antibody detects POLR2A only when phosphorylated at Ser1619. Validated for WB, IHC, IF and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
  • Product Name
    POLR2A (Phospho-Ser1619) Antibody
  • Catalog No
    A0082
  • Storage/Stability
    Stable at -20°C for at least 1 year.
  • Applications
    WB, IHC, IF, ELISA
  • Clonality
    Polyclonal
  • Host Species
    Rabbit
  • Reactivity
    Human, Mouse, Rat
  • Application Dilutions
    WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000
  • Database Links
    Gene ID: 5430, Swiss-Prot #: P24928, OMIM #: 180660, Unigene #: Hs.270017
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human POLR2A around the phosphorylation site of Ser1619.
  • Immunogen Range
    1585-1634
  • Modification Sites
    H: S1619 M: S1619 R: S1619
  • MW
    217 kDa
  • NCBI Gene Symbol
    RPB1
  • Physical Form
    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Purification
    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
  • Specificity
    POLR2A (Phospho-Ser1619) Antibody detects endogenous levels of POLR2A only when phosphorylated at Ser1619.
  • Synonyms
    DNA-directed RNA polymerase II largest subunit, RNA pol II largest subunit, RNA polymerase II 220 kd subunit, RPB1
  • Target Modification
    Phospho
Application Images
Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using POLR2A (Phospho-Ser1619) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using POLR2A (Phospho-Ser1619) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of HeLa cells treated with PMA 125ng/ml 30', using POLR2A (Phospho-Ser1619) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of VEC using p-Rpb1 (S1619) antibody. Antibody was diluted at 1:2000
Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using POLR2A (Phospho-Ser1619) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using POLR2A (Phospho-Ser1619) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of HeLa cells treated with PMA 125ng/ml 30', using POLR2A (Phospho-Ser1619) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of VEC using p-Rpb1 (S1619) antibody. Antibody was diluted at 1:2000