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PI3-kinase p85-alpha/gamma (Phospho-Tyr467/199) Antibody

Catalog No : A1224
  • Applications
    WB, IF, ELISA
  • Reactivity
    Human, Mouse, Rat
  • Size
    50ul
  • Price
    $195.00
Anti-PI3-kinase p85-alpha/gamma (Phospho-Tyr467/199) polyclonal Antibody detects PI3-kinase p85-alpha/gamma only when phosphorylated at Tyr467/199. Validated for WB, IF and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
  • Product Name
    PI3-kinase p85-alpha/gamma (Phospho-Tyr467/199) Antibody
  • Catalog No
    A1224
  • Storage/Stability
    Stable at -20°C for at least 1 year.
  • Applications
    WB, IF, ELISA
  • Clonality
    Polyclonal
  • Host Species
    Rabbit
  • Reactivity
    Human, Mouse, Rat
  • Application Dilutions
    WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:10000
  • Database Links
    Gene ID: 5295, Swiss-Prot #: P27986/Q92569, OMIM #: 171833/606076, Unigene #: Hs.132225/Hs.604502/Hs.655387
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human PI3-kinase p85-alpha/gamma around the phosphorylation site of Tyr467/199.
  • Immunogen Range
    436-485
  • Modification Sites
    H: Y467/Y199 M: Y467/Y199 R: Y467/Y199
  • MW
    83 kDa
  • NCBI Gene Symbol
    PIK3R1
  • Physical Form
    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Purification
    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
  • Specificity
    PI3-kinase p85-alpha/gamma (Phospho-Tyr467/199) Antibody detects endogenous levels of PI3-kinase p85-alpha/gamma only when phosphorylated at Tyr467/199.
  • Synonyms
    GRB1, P85A, PI3-kinase p85-alpha subunit, PI3K, PI3K p85-alpha, Phosphatidylinositol 3-kinase regulatory alpha subunit, PtdIns-3-kinase p85-alpha, P55G, PI3-kinase p85-gamma subunit, PI3K p85-gamma, Phosphatidylinositol 3-kinase regulatory gamma subunit
  • Target Modification
    Phospho
Application Images
Western blot analysis of lysates from COS7 cells treated with H2O2 100uM 30', using PI3-kinase p85-alpha/gamma (Phospho-Tyr467/199) Antibody. The lane on the right is blocked with the phospho peptide.
Immunofluorescence analysis of NIH/3T3 cells, using PI3-kinase p85-alpha/gamma (Phospho-Tyr467/199) Antibody. The picture on the right is blocked with the phospho peptide.
Western Blot analysis of various cells using primary antibody diluted at 1:1000(4°C ON). Secondary antibody?Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour)
Western Blot analysis of COS7 cells using Phospho-PI 3-kinase p85/p55 (Y467/199) Antibody diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. PI 3-kinase p85/p55 (phospho Tyr467/199) Antibody was diluted at 1:200(4°C,ON). Negative control was defined by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. PI 3-kinase p85/p55 (phospho Tyr467/199) Antibody was diluted at 1:200(4°C,ON). Negative control was defined by secondary antibody only.
Immunofluorescence analysis of rat-spleen tissue. PI 3-kinase p85/p55 (phospho Tyr467/199) Antibody(red) was diluted at 1:200(4°C,ON). Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of human-lung tissue. PI 3-kinase p85/p55 (phospho Tyr467/199) Antibody(red) was diluted at 1:200(4°C,ON). Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Western blot analysis of lysates from COS7 cells treated with H2O2 100uM 30', using PI3-kinase p85-alpha/gamma (Phospho-Tyr467/199) Antibody. The lane on the right is blocked with the phospho peptide.
Immunofluorescence analysis of NIH/3T3 cells, using PI3-kinase p85-alpha/gamma (Phospho-Tyr467/199) Antibody. The picture on the right is blocked with the phospho peptide.
Western Blot analysis of various cells using primary antibody diluted at 1:1000(4°C ON). Secondary antibody?Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour)
Western Blot analysis of COS7 cells using Phospho-PI 3-kinase p85/p55 (Y467/199) Antibody diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. PI 3-kinase p85/p55 (phospho Tyr467/199) Antibody was diluted at 1:200(4°C,ON). Negative control was defined by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. PI 3-kinase p85/p55 (phospho Tyr467/199) Antibody was diluted at 1:200(4°C,ON). Negative control was defined by secondary antibody only.
Immunofluorescence analysis of rat-spleen tissue. PI 3-kinase p85/p55 (phospho Tyr467/199) Antibody(red) was diluted at 1:200(4°C,ON). Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of human-lung tissue. PI 3-kinase p85/p55 (phospho Tyr467/199) Antibody(red) was diluted at 1:200(4°C,ON). Picture A:Target. Picture B: DAPI. Picture C: merge of A+B