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PI3-kinase p85-alpha (Phospho-Tyr607) Antibody

Catalog No : A1225
  • Applications
    WB, IHC, ELISA
  • Reactivity
    Human, Mouse, Rat
  • Size
    50ul
  • Price
    $195.00
Anti-PI3-kinase p85-alpha (Phospho-Tyr607) polyclonal Antibody detects PI3-kinase p85-alpha only when phosphorylated at Tyr607. Validated for WB, IHC and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
  • Product Name
    PI3-kinase p85-alpha (Phospho-Tyr607) Antibody
  • Catalog No
    A1225
  • Storage/Stability
    Stable at -20°C for at least 1 year.
  • Applications
    WB, IHC, ELISA
  • Clonality
    Polyclonal
  • Host Species
    Rabbit
  • Reactivity
    Human, Mouse, Rat
  • Application Dilutions
    WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
  • Database Links
    Gene ID: 5295, Swiss-Prot #: P27986, OMIM #: 171833, Unigene #: Hs.132225/Hs.604502
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human PI3-kinase p85-alpha around the phosphorylation site of Tyr607.
  • Immunogen Range
    573-622
  • Modification Sites
    H: Y607 M: Y607 R: Y607
  • MW
    83 kDa
  • NCBI Gene Symbol
    PIK3R1
  • Physical Form
    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Purification
    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
  • Specificity
    PI3-kinase p85-alpha (Phospho-Tyr607) Antibody detects endogenous levels of PI3-kinase p85-alpha only when phosphorylated at Tyr607.
  • Synonyms
    Phosphatidylinositol 3-kinase regulatory subunit alpha, PI3-kinase p85 subunit alpha, PtdIns-3-kinase p85-alpha, PI3K, PIK3R1, GRB1
  • Target Modification
    Phospho
Application Images
Western blot analysis of lysates from rat kidney, using PI3-kinase p85-alpha (Phospho-Tyr607) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human brain, using PI3-kinase p85-alpha (Phospho-Tyr607) Antibody. The picture on the right is blocked with the phospho peptide.
Western Blot analysis of various cells using Phospho-PI 3-kinase p85alpha (Y607) Antibody diluted at 1:1000
Western Blot analysis of KB cells using Phospho-PI 3-kinase p85alpha (Y607) Antibody diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded Human-liver tissue. PI 3-kinase p85alpha (phospho Tyr607) Antibody was diluted at 1:200(4°C,ON). Negative control was defined by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. PI 3-kinase p85alpha (phospho Tyr607) Antibody was diluted at 1:200(4°C,ON). Negative control was defined by secondary antibody only.
Immunofluorescence analysis of rat-heart tissue. PI 3-kinase p85alpha (phospho Tyr607) Antibody(red) was diluted at 1:200(4°C,ON). Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of rat-heart tissue. PI 3-kinase p85alpha (phospho Tyr607) Antibody(red) was diluted at 1:200(4°C,ON). Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Western blot analysis of lysates from rat kidney, using PI3-kinase p85-alpha (Phospho-Tyr607) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human brain, using PI3-kinase p85-alpha (Phospho-Tyr607) Antibody. The picture on the right is blocked with the phospho peptide.
Western Blot analysis of various cells using Phospho-PI 3-kinase p85alpha (Y607) Antibody diluted at 1:1000
Western Blot analysis of KB cells using Phospho-PI 3-kinase p85alpha (Y607) Antibody diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded Human-liver tissue. PI 3-kinase p85alpha (phospho Tyr607) Antibody was diluted at 1:200(4°C,ON). Negative control was defined by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. PI 3-kinase p85alpha (phospho Tyr607) Antibody was diluted at 1:200(4°C,ON). Negative control was defined by secondary antibody only.
Immunofluorescence analysis of rat-heart tissue. PI 3-kinase p85alpha (phospho Tyr607) Antibody(red) was diluted at 1:200(4°C,ON). Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of rat-heart tissue. PI 3-kinase p85alpha (phospho Tyr607) Antibody(red) was diluted at 1:200(4°C,ON). Picture A:Target. Picture B: DAPI. Picture C: merge of A+B