Anti-Nibrin (Phospho-Ser278) polyclonal Antibody detects Nibrin only when phosphorylated at Ser278. Validated for WB, IF and ELISA. Reactive for Human. AssayBiotechnology an original antibody manufacturer.
Details
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Product Name
Nibrin (Phospho-Ser278) Antibody
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Catalog No
A1221
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Storage/Stability
Stable at -20°C for at least 1 year.
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Applications
WB, IF, ELISA
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Clonality
Polyclonal
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Host Species
Rabbit
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Reactivity
Human
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Application Dilutions
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000
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Database Links
Gene ID: 4683, Swiss-Prot #: O60934, OMIM #: 114480/251260/602667/609135, Unigene #: Hs.492208
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Immunogen
The antiserum was produced against synthesized peptide derived from human Nibrin around the phosphorylation site of Ser278.
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Immunogen Range
251-300
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Modification Sites
H: S278
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MW
84 kDa
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NCBI Gene Symbol
NBN
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Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
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Specificity
Nibrin (Phospho-Ser278) Antibody detects endogenous levels of Nibrin only when phosphorylated at Ser278.
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Synonyms
Cell cycle regulatory protein P95, NBN, NBS, NIBRIN, NIJMEGEN BREAKAGE syndrome protein 1, p95-NBS1
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Target Modification
Phospho
Application Images
Immunofluorescence analysis of NIH/3T3 cells, using Nibrin (Phospho-Ser278) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from HUVEC cells treated with Forskolin 40nM 30', using Nibrin (Phospho-Ser278) Antibody. The lane on the right is blocked with the phospho peptide.
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Immunofluorescence analysis of NIH/3T3 cells, using Nibrin (Phospho-Ser278) Antibody. The picture on the right is blocked with the phospho peptide. |
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Western blot analysis of lysates from HUVEC cells treated with Forskolin 40nM 30', using Nibrin (Phospho-Ser278) Antibody. The lane on the right is blocked with the phospho peptide. |
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