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NF-kappaB p105/p50 (Phospho-Ser927) Antibody

Catalog No : A0021
  • Applications
    WB, IHC, IF, ELISA
  • Reactivity
    Human, Mouse
  • Size
    50ul
  • Price
    $195.00
Anti-NF-kappaB p105/p50 (Phospho-Ser927) polyclonal Antibody detects NF-kappaB p105/p50 only when phosphorylated at Ser927. Validated for WB, IHC, IF and ELISA. Reactive for Human, Mouse. AssayBiotechnology an original antibody manufacturer.
Details
  • Product Name
    NF-kappaB p105/p50 (Phospho-Ser927) Antibody
  • Catalog No
    A0021
  • Storage/Stability
    Stable at -20°C for at least 1 year.
  • Applications
    WB, IHC, IF, ELISA
  • Clonality
    Polyclonal
  • Host Species
    Rabbit
  • Reactivity
    Human, Mouse
  • Application Dilutions
    WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000
  • Database Links
    Gene ID: 4790, Swiss-Prot #: P19838, OMIM #: 164011, Unigene #: Hs.654408
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human NF-kappaB p105/p50 around the phosphorylation site of Ser927.
  • Immunogen Range
    896-945
  • Modification Sites
    H: S927 M: S930
  • MW
    105 kDa
  • NCBI Gene Symbol
    NFKB1
  • Physical Form
    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Purification
    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
  • Specificity
    NF-kappaB p105/p50 (Phospho-Ser927) Antibody detects endogenous levels of NF-kappaB p105/p50 only when phosphorylated at Ser927.
  • Synonyms
    DNA-binding factor KBF1, EBP-1, NF-kappa-B1 p84/NF-kappa-B1 p98, NFKB1, NFkB-p50, Nuclear factor NF-kappa-B p105 subunit, nuclear factor NF-kappa-B p50 subunit
  • Target Modification
    Phospho
Application Images
Western blot analysis of lysates from HeLa cells treated with LPS 100ng/ml 30', using NF-kappaB p105/p50 (Phospho-Ser927) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NF-kappaB p105/p50 (Phospho-Ser927) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of HeLa cells treated with EGF 200nM 5', using NF-kappaB p105/p50 (Phospho-Ser927) Antibody. The picture on the right is blocked with the phospho peptide.
Western Blot analysis of various cells using Phospho-NFkappaB-p105 (S927) Antibody
Western blot analysis of lysates from HeLa cells treated with LPS 100ng/ml 30', using NF-kappaB p105/p50 (Phospho-Ser927) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NF-kappaB p105/p50 (Phospho-Ser927) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of HeLa cells treated with EGF 200nM 5', using NF-kappaB p105/p50 (Phospho-Ser927) Antibody. The picture on the right is blocked with the phospho peptide.
Western Blot analysis of various cells using Phospho-NFkappaB-p105 (S927) Antibody