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NF-kB p65 (Phospho-Ser529) Fluorometric Cell-Based ELISA Kit

Catalog No : FLUO-CBP1091
  • Reactivity
    Human , Mouse , Rat
  • Assay Type
    Cell-Based ELISA
  • Size
    2 Kits
  • Price
    Inquire
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The NF-kB p65 (Phospho-Ser529) Fluorometric Cell-Based Phospho ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor NF-kB p65 (Phospho-Ser529) protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated NF-kB p65 (Phospho-Ser529) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals, or activators have on RELA phosphorylation.
Details
  • Product Name
  • Catalog No
  • Detection Method
    Fluorometric
  • Dynamic Range
    > 5000 Cells
  • Storage/Stability
    4°C/6 Months
  • Reactivity
    Human, Mouse, Rat
  • Assay Type
    Cell-Based ELISA
  • Database Links
    Gene ID: 5970, UniProt ID: Q04206, OMIM #: 164014, Unigene #: Hs.502875
  • Format
    Two 96-Well Plates
  • Manual (PDF)
    <a href='image/catalog/assbiotech/FLUO-CBP1091-2018.pdf'>Download</a>
  • NCBI Gene Symbol
    RELA
  • Sub Type
    Phospho
  • Synonyms
    NFKB3, nuclear factor NF-kappa-B p65 subunit, p65, RELA, TF65, transcription factor p65
  • TargetName
    Phospho-NF-κB p65 (Ser529)
Application Images
Image 1 Western blot analysis of extracts from HeLa and Jurkat cells, using NF-kappaB p65 (Phospho-Ser529) Antibody.
Image 2 HeLa cells starved for 24 hrs. and stimulated with PMA 200nM for 1 hr..
Image 3 An illustrated breakdown of the colorimetric Non-Phospho Cell-Based ELISA assay principles.
Image 4 A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits.
Image 5 The Anti-GAPDH Antibody is a mouse monoclonal antibody used as a positive control in Assay Biotechnology's Cell-Based ELISA kits. It was tested on Western Blots with the tissue lysates from human, mouse, and rat for specificity.
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