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MMP3 Assay Kit - Fluorometric

Catalog No : EZ-0531
  • Size
    100 Assays
  • Price
    $295.00
In this assay, a fluorescence resonance energy transfer (FRET) peptide is used to quantify MMP-3 activity. The assay is more selective for MMP-3 hydrolysis over other MMP enzymes and can be used to screen for MMP-3 inhibitors when purified MMP-3 enzyme is used. Fluorescence of intact FRET peptide is kept low with a quencher, but upon cleavage into two separate fragements by MMP-3, green fluorescent is recovered. Signals increase with increasing MMP-3 activity, and can be read by fluorescence microplate reader, as well as almost all common fluorescence instruments equipped with Argon laser and FITC filter set with excitation at 490 nm and emission at 525 nm. 
Details
  • Product Name
    MMP3 Assay Kit - Fluorometric
  • Catalog No
    EZ-0531
  • Detection Method
    Fluorometric
  • Storage/Stability
    -20°, C/3 Months
  • Fluorometric Readout [Ex (nm), Em (nm)]
    490, 525
  • Manual
    Download
  • Tar Name
    MMP3
Application Images
The MMP3 Assay Kit - Fluorometric allows for the detection and quantification of endogenous MMP3 in cell lysates, sera, and plasma.
The MMP3 Assay Kit - Fluorometric allows for the detection and quantification of endogenous MMP3 in cell lysates, sera, and plasma.