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MEK1 (Phospho-Thr291) Antibody

Catalog No : A7148
  • Applications
    WB, ELISA
  • Reactivity
    Human, Mouse, Rat
  • Size
    50ul
  • Price
    $195.00
Anti-MEK1 (Phospho-Thr291) polyclonal Antibody detects MEK1 only when phosphorylated at Thr291. Validated for WB and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
  • Product Name
    MEK1 (Phospho-Thr291) Antibody
  • Catalog No
    A7148
  • Storage/Stability
    Stable at -20°C for at least 1 year.
  • Applications
    WB, ELISA
  • Clonality
    Polyclonal
  • Host Species
    Rabbit
  • Reactivity
    Human, Mouse, Rat
  • Application Dilutions
    WB: 1:500~1:1000 ELISA: 1:10000
  • Database Links
    Gene ID: 5604, Swiss-Prot #: Q02750, OMIM #: 115150/176872, Unigene #: Hs.145442
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human MEK1 around the phosphorylation site of Thr291.
  • Immunogen Range
    261-310
  • Modification Sites
    H: T292 M: T292 R: T292
  • MW
    43 kDa
  • NCBI Gene Symbol
    MAP2K1
  • Physical Form
    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Purification
    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
  • Specificity
    MEK1 (Phospho-Thr291) Antibody detects endogenous levels of MEK1 only when phosphorylated at Thr291.
  • Synonyms
    Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAP kinase kinase 1, MAP2K1, MAPK/ERK kinase 1, MAPKK 1, MAPKK1, MEK1, MP2K1, PRKMK1, kinase MEK1
  • Target Modification
    Phospho
Application Images
Image 1 Western blot analysis of lysates from K562 cells, using MEK1 (Phospho-Thr291) Antibody. The lane on the right is blocked with the phospho peptide.
Image 2 Western Blot analysis of 293 cells using Phospho-MEK-1 (T292) Antibody diluted at 1:1000
Image 3 Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,ON). Negative control (right) obtaned from antibody was pre-absorbed by immunogen peptide.