Anti-MEK1 (Phospho-Thr286) polyclonal Antibody detects MEK1 only when phosphorylated at Thr286. Validated for WB, IHC and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
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Product Name
MEK1 (Phospho-Thr286) Antibody
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Catalog No
A0681
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Storage/Stability
Stable at -20°C for at least 1 year.
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Applications
WB, IHC, ELISA
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Clonality
Polyclonal
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Host Species
Rabbit
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Reactivity
Human, Mouse, Rat
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Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000
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Database Links
Gene ID: 5604, Swiss-Prot #: Q02750, OMIM #: 115150/176872, Unigene #: Hs.145442
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Immunogen
The antiserum was produced against synthesized peptide derived from human MEK1 around the phosphorylation site of Thr286.
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Immunogen Range
252-301
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Modification Sites
H: T286 M: T286 R: T286
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MW
43 kDa
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NCBI Gene Symbol
MAP2K1
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Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
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Specificity
MEK1 (Phospho-Thr286) Antibody detects endogenous levels of MEK1 only when phosphorylated at Thr286.
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Synonyms
Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAP kinase kinase 1, MAP2K1, MAPK/ERK kinase 1, MAPKK 1, MAPKK1, MEK1, MP2K1, PRKMK1, kinase MEK1
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Target Modification
Phospho
Application Images
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MEK1 (Phospho-Thr286) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from K562 cells, using MEK1 (Phospho-Thr286) Antibody. The lane on the right is blocked with the phospho peptide.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MEK1 (Phospho-Thr286) Antibody. The picture on the right is blocked with the phospho peptide. |
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Western blot analysis of lysates from K562 cells, using MEK1 (Phospho-Thr286) Antibody. The lane on the right is blocked with the phospho peptide. |
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