Anti-MEK1/2 (Phospho-Ser221) polyclonal Antibody detects MEK1/2 only when phosphorylated at Ser221. Validated for WB, IHC and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
MEK1/2 (Phospho-Ser221) Antibody
Stable at -20°C for at least 1 year.
WB, IHC, ELISA
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Gene ID: 5604/5605, Swiss-Prot #: Q02750/P36507, OMIM #: 115150/176872/115150/601263, Unigene #: Hs.145442/Hs.465627
The antiserum was produced against synthesized peptide derived from human MEK1/2 around the phosphorylation site of Ser221.
H: S222/226 M: S222/226 R: S222/226
NCBI Gene Symbol
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
MEK1/2 (Phospho-Ser221) Antibody detects endogenous levels of MEK1/2 only when phosphorylated at Ser221.
Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAP kinase kinase 1, MAP2K1, MAPK/ERK kinase 1, MAPKK 1, MAPKK1, MEK1, MP2K1, PRKMK1, kinase MEK1
||Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MEK1/2 (Phospho-Ser221) Antibody. The picture on the right is blocked with the phospho peptide.
||Western blot analysis of lysates from K562 cells treated with serum 20% 15' and Jurkat cells treated with EGF, using MEK1/2 (Phospho-Ser221) Antibody. The lane on the right is blocked with the phospho peptide.