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MEK1/2 (Phospho-Ser217) Antibody

Catalog No : A7146
  • Applications
    WB, IHC, ELISA
  • Reactivity
    Human, Mouse, Rat
  • Size
    50ul
  • Price
    $195.00
Anti-MEK1/2 (Phospho-Ser217) polyclonal Antibody detects MEK1/2 only when phosphorylated at Ser217. Validated for WB, IHC and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
  • Product Name
    MEK1/2 (Phospho-Ser217) Antibody
  • Catalog No
    A7146
  • Storage/Stability
    Stable at -20°C for at least 1 year.
  • Applications
    WB, IHC, ELISA
  • Clonality
    Polyclonal
  • Host Species
    Rabbit
  • Reactivity
    Human, Mouse, Rat
  • Application Dilutions
    WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:4000
  • Database Links
    Gene ID: 5604/5605, Swiss-Prot #: Q02750/P36507, OMIM #: 115150/176872/115150/601263, Unigene #: Hs.145442/Hs.465627
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human MEK1/2 around the phosphorylation site of Ser217.
  • Immunogen Range
    189-238
  • Modification Sites
    H: S218/222 M: S218/222 R: S218/222
  • MW
    43 kDa
  • NCBI Gene Symbol
    MAP2K2
  • Physical Form
    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Purification
    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
  • Specificity
    MEK1/2 (Phospho-Ser217) Antibody detects endogenous levels of MEK1/2 only when phosphorylated at Ser217.
  • Synonyms
    Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAP kinase kinase 1, MAP2K1, MAPK/ERK kinase 1, MAPKK 1, MAPKK1, MEK1, MP2K1, PRKMK1, kinase MEK1
  • Target Modification
    Phospho
Application Images
Image 1 Western blot analysis of lysates from 293 cells treated with PMA 125ng/ml 30', using MEK1/2 (Phospho-Ser217) Antibody. The lane on the right is blocked with the phospho peptide.
Image 2 Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MEK1/2 (Phospho-Ser217) Antibody. The picture on the right is blocked with the phospho peptide.
Image 3 Western Blot analysis of various cells using Phospho-MEK-1/2 (S218/222) Antibody
Image 4 Western Blot analysis of 293 cells using Phospho-MEK-1/2 (S218/222) Antibody
Image 5 Immunohistochemical analysis of paraffin-embedded Human-liver tissue. MEK-1/2 (phospho Ser218/222) Antibody was diluted at 1:200(4°C,ON). Negative control was defined by secondary antibody only.
Image 6 Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. MEK-1/2 (phospho Ser218/222) Antibody was diluted at 1:200(4°C,ON). Negative control was defined by secondary antibody only.