Anti-MEF2A (Phospho-Ser408) polyclonal Antibody detects MEF2A only when phosphorylated at Ser408. Validated for WB, IHC, IF and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
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Product Name
MEF2A (Phospho-Ser408) Antibody
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Catalog No
A0019
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Storage/Stability
Stable at -20°C for at least 1 year.
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Applications
WB, IHC, IF, ELISA
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Clonality
Polyclonal
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Host Species
Rabbit
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Reactivity
Human, Mouse, Rat
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Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000
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Database Links
Gene ID: 4205, Swiss-Prot #: Q02078, OMIM #: 600660/608320, Unigene #: Hs.268675
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Immunogen
The antiserum was produced against synthesized peptide derived from human MEF2A around the phosphorylation site of Ser408.
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Immunogen Range
374-423
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Modification Sites
H: S408 M: S406 R: S400
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MW
54 kDa
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NCBI Gene Symbol
MEF2A
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Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
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Specificity
MEF2A (Phospho-Ser408) Antibody detects endogenous levels of MEF2A only when phosphorylated at Ser408.
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Synonyms
MEF2, Myocyte-specific enhancer factor 2A, Serum response factor-like protein 1
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Target Modification
Phospho
Application Images
Western blot analysis of lysates from HeLa cells treated with PMA 125ng/ml 30', using MEF2A (Phospho-Ser408) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MEF2A (Phospho-Ser408) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of HeLa cells treated with PMA 125ng/ml 30', using MEF2A (Phospho-Ser408) Antibody. The picture on the right is blocked with the phospho peptide.
Western Blot analysis of various cells using Phospho-MEF-2 (S408)
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Western blot analysis of lysates from HeLa cells treated with PMA 125ng/ml 30', using MEF2A (Phospho-Ser408) Antibody. The lane on the right is blocked with the phospho peptide. |
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MEF2A (Phospho-Ser408) Antibody. The picture on the right is blocked with the phospho peptide. |
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Immunofluorescence analysis of HeLa cells treated with PMA 125ng/ml 30', using MEF2A (Phospho-Ser408) Antibody. The picture on the right is blocked with the phospho peptide. |
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Western Blot analysis of various cells using Phospho-MEF-2 (S408) |
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