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MDR Assay Kit - Fluorometric

Catalog No : EZ-0539
  • Size
    100 Assays
  • Price
    $195.00
This assay is ideal for high-throughput screening of MDR pump inhibitors or identifying cells that have high levels of MDR pump activities. A fluorescent MDR indicator is used to assay the activity of the MDR1 and MRP1 MDR. Hydrophobic fluorescent dye molecules rapidly penetrate the cell membranes and become trapped in the cells. After a short incubation, the intracellular free dye concentration will significantly increase. This dye is extruded by the MDR transported in the cells that express MDR1 or MRP1, decreasing cellular fluorescence intensity. However, when extrusion is blocked by agent that interferes with the pump activities, the cellular fluorescence intensity will elevate significantly. 
Details
  • Product Name
    MDR Assay Kit - Fluorometric
  • Catalog No
    EZ-0539
  • Detection Method
    Fluorometric
  • Storage/Stability
    -20°, C/3 Months
  • Fluorometric Readout [Ex (nm), Em (nm)]
    490, 525
  • Manual
    Download
  • Range Detection
    0.0 mM - 100 mM
  • Tar Name
    MDR
Application Images
The MDR Assay Kit - Fluorometric allows for the detection and quantification of endogenous MDR within the range of 0.0 to 100 µM MDR in cell lysates, sera, and plasma.
The MDR Assay Kit - Fluorometric allows for the detection and quantification of endogenous MDR within the range of 0.0 to 100 µM MDR in cell lysates, sera, and plasma.