Anti-MAPKAPK2 (Phospho-Thr334) polyclonal Antibody detects MAPKAPK2 only when phosphorylated at Thr334. Validated for WB, IHC and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
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Product Name
MAPKAPK2 (Phospho-Thr334) Antibody
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Catalog No
A0018
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Storage/Stability
Stable at -20°C for at least 1 year.
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Applications
WB, IHC, ELISA
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Clonality
Polyclonal
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Host Species
Rabbit
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Reactivity
Human, Mouse, Rat
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Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
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Database Links
Gene ID: 9261, Swiss-Prot #: P49137, OMIM #: 602006, Unigene #: Hs.643566
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Immunogen
The antiserum was produced against synthesized peptide derived from human MAPKAPK2 around the phosphorylation site of Thr334.
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Immunogen Range
300-349
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Modification Sites
H: T334 M: T320 R: T320
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MW
45 kDa
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NCBI Gene Symbol
MAPKAPK2
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Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
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Specificity
MAPKAPK2 (Phospho-Thr334) Antibody detects endogenous levels of MAPKAPK2 only when phosphorylated at Thr334.
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Synonyms
MAP kinase-activated protein kinase 2, MAPK-activated protein kinase 2, MAPK2, MAPKAP kinase 2, MAPKAPK-2, MAPKAPK2, RPS6KC1, kinase MAPKAPK2
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Target Modification
Phospho
Application Images
Western blot analysis of lysates from NIH/3T3 cells, using MAPKAPK2 (Phospho-Thr334) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human brain, using MAPKAPK2 (Phospho-Thr334) Antibody. The picture on the right is blocked with the phospho peptide.
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,ON). Negative control (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Western blot analysis of lysates from NIH/3T3 cells, using MAPKAPK2 (Phospho-Thr334) Antibody. The lane on the right is blocked with the phospho peptide. |
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Immunohistochemistry analysis of paraffin-embedded human brain, using MAPKAPK2 (Phospho-Thr334) Antibody. The picture on the right is blocked with the phospho peptide. |
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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,ON). Negative control (right) obtaned from antibody was pre-absorbed by immunogen peptide. |
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