| This assay measures activity of monoamine oxidase as well as plasma amine oxidase (PAO), or semicarbazide-sensitive amine oxidase (SSAO), in biological samples including blood samples. In the assay, MAO oxidizes monoamine to produce hydrogen peroxide, which then reacts with a proprietary fluorescent dye in presence of HRP to produce a red fluorescent product, resorufin. No separations step is required. Signals increase with increasing MAO activity, and can be read with both a fluorescence microplate reader at excitation at 540 and emission at 590 nm or an absorbance microplate reader at 576 nm. |
| Image 1 | 
|
The MAO Assay Kit - Fluorometric allows for the detection and quantification of endogenous MAO within the range of ≥10 mU/ml in cell lysates, sera, and plasma. |
| Image 2 | 
|
