This assay detects
activity of lysyl oxidase and is more sensitive than currently available
assays. It can readily detect lysyl oxidase activity in cell extracts or
solutions and eliminates interference from biological samples. In the assay,
lysyl oxidase reacts with proprietary LOX substrate to form hydrogen
peroxide. A HRP-coupled reaction then converts the hydrogen peroxide and ADHP
substrate into a red fluorescent product, resorufin. Signals increase with
increasing lysyl oxidase activity, and can be read with fluorescence
microplate reader at excitation at 520 and emission at 590 nm or an
absorbance microplate reader at 576 nm. |