| This assay detects activity of lysyl oxidase and is more sensitive than currently available assays. It can readily detect lysyl oxidase activity in cell extracts or solutions and eliminates interference from biological samples. In the assay, lysyl oxidase reacts with proprietary LOX substrate to form hydrogen peroxide. A HRP-coupled reaction then converts the hydrogen peroxide and ADHP substrate into a red fluorescent product, resorufin. Signals increase with increasing lysyl oxidase activity, and can be read with fluorescence microplate reader at excitation at 520 and emission at 590 nm or an absorbance microplate reader at 576 nm. |
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The Lysyl Oxidase Asay Kit - Fluorometric allows for the detection and quantification of endogenous Lysyl Oxidase within the range of ≥40 ng lysyl oxidase/well in cell lysates, sera, and plasma. |
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EZ-0501
EZ-0502
EZ-0503
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