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Luciferase Assay Kit - Luminometric

Catalog No : EZ-0529
  • Size
    100 Assays
  • Price
    $145.00
This protocol is compatible with HTS liquid handling instruments and is highly sensitive, allowing us to analyze samples with low detection to study gene regulation and function in a quick and simple manner. This mix-and-read assay can quantify luciferase activity in live cells and cell extracts when the proprietary luminogenic formulation interacts with luciferase, forming a luminescent product. The first step involves activation of protein by ATP to produce a reactive mixed anhydride intermediate. This intermediate reacts with oxygen in the second step to create a transient dioxetane, which quickly breaks down to the oxidized products of oxyluciferin and carbon dioxide. Along with these products, the reaction yields a burst of light at 560 nm, a “glow-type” signal that has a half-life of two to four hours. 
Details
  • Product Name
    Luciferase Assay Kit - Luminometric
  • Catalog No
    EZ-0529
  • Detection Method
    Luminometric
  • Storage/Stability
    -20°, C/3 Months
  • Manual
    Download
  • Range Detection
    ?0.1 pg Luciferase/Well
  • Tar Name
    Luciferase
Application Images
The Luciferase Assay Kit - Luminometric allows for the detection and quantification of endogenous Luciferase within the range of ≥0.1 pg luciferase/well in cell lysates, sera, and plasma.
The Luciferase Assay Kit - Luminometric allows for the detection and quantification of endogenous Luciferase within the range of ≥0.1 pg luciferase/well in cell lysates, sera, and plasma.