The Keratin 8 (Phospho-Ser73) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect Keratin 8 protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated Keratin 8 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Keratin 8 phosphorylation.
Details
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Product Name
Keratin 8 (Phospho-Ser73) Colorimetric Cell-Based ELISA Kit
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Catalog No
CBP1017
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Detection Method
Colorimetric 450 nm
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Dynamic Range
> 5000 Cells
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Storage/Stability
4°C/6 Months
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Reactivity
Human, Mouse
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Assay Type
Cell-Based
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Database Links
Gene ID: 3856, UniProt ID: P05787, OMIM #: 148060/215600, Unigene #: Hs.533782/Hs.708445
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Format
2 x 96-Well Microplates
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Manual (PDF)
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NCBI Gene Symbol
KRT8
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Sub Type
Phospho
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Synonyms
CK 8, CK8, CYK8, Cytokeratin endo A, K2C8, K8, KRT2-8, KRT8, Keratin type II cytoskeletal 8, cytokeratin 8
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TargetName
Keratin 8 (Phospho-Ser73)
Application Images
Western blot analysis of extracts from 293 cells treated with Etoposide 25uM 60', using Keratin 8 (Phospho-Ser73) Antibody.
An illustrated breakdown of the colorimetric Non-Phospho Cell-Based ELISA assay principles.
A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits.
The Anti-GAPDH Antibody is a mouse monoclonal antibody used as a positive control in Assay Biotechnology's Cell-Based ELISA kits. It was tested on Western Blots with the tissue lysates from human, mouse, and rat for specificity.
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Western blot analysis of extracts from 293 cells treated with Etoposide 25uM 60', using Keratin 8 (Phospho-Ser73) Antibody. |
|
An illustrated breakdown of the colorimetric Non-Phospho Cell-Based ELISA assay principles. |
|
A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits. |
|
The Anti-GAPDH Antibody is a mouse monoclonal antibody used as a positive control in Assay Biotechnology's Cell-Based ELISA kits. It was tested on Western Blots with the tissue lysates from human, mouse, and rat for specificity. |
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