Anti-Keratin 8 (Phospho-Ser73) polyclonal Antibody detects Keratin 8 only when phosphorylated at Ser73. Validated for WB, IHC and ELISA. Reactive for Human, Mouse. AssayBiotechnology an original antibody manufacturer.
Details
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Product Name
Keratin 8 (Phospho-Ser73) Antibody
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Catalog No
A0017
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Storage/Stability
Stable at -20°C for at least 1 year.
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Applications
WB, IHC, ELISA
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Clonality
Polyclonal
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Host Species
Rabbit
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Reactivity
Human, Mouse
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Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000
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Database Links
Gene ID: 3856, Swiss-Prot #: P05787, OMIM #: 148060/215600, Unigene #: Hs.533782/Hs.708445
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Immunogen
The antiserum was produced against synthesized peptide derived from human Keratin 8 around the phosphorylation site of Ser73.
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Immunogen Range
41-90
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Modification Sites
H: S73 M: S79
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MW
53 kDa
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NCBI Gene Symbol
KRT8
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Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
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Specificity
Keratin 8 (Phospho-Ser73) Antibody detects endogenous levels of Keratin 8 only when phosphorylated at Ser73.
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Synonyms
CK 8, CK8, CYK8, Cytokeratin endo A, K2C8, K8, KRT2-8, KRT8, Keratin, type II cytoskeletal 8, cytokeratin 8
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Target Modification
Phospho
Application Images
Western blot analysis of lysates from 293 cells treated with Etoposide 25uM 60', using Keratin 8 (Phospho-Ser73) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Keratin 8 (Phospho-Ser73) Antibody. The picture on the right is blocked with the phospho peptide.
Western Blot analysis of various cells using Phospho-Cytokeratin 8 (S73) Antibody diluted at 1:500
Western Blot analysis of 293 cells using Phospho-Cytokeratin 8 (S73) Antibody diluted at 1:500
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,ON). Negative control (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Western blot analysis of lysates from 293 cells treated with Etoposide 25uM 60', using Keratin 8 (Phospho-Ser73) Antibody. The lane on the right is blocked with the phospho peptide. |
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Keratin 8 (Phospho-Ser73) Antibody. The picture on the right is blocked with the phospho peptide. |
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Western Blot analysis of various cells using Phospho-Cytokeratin 8 (S73) Antibody diluted at 1:500 |
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Western Blot analysis of 293 cells using Phospho-Cytokeratin 8 (S73) Antibody diluted at 1:500 |
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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,ON). Negative control (right) obtaned from antibody was pre-absorbed by immunogen peptide. |
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