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Keratin 8 (Phospho-Ser73) Antibody

Catalog No : A0017
  • Applications
    WB , IHC , ELISA
  • Reactivity
    Human , Mouse
  • Application Dilutions
    WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000
  • Size
    50ul
  • Price
    $195.00
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Anti-Keratin 8 (Phospho-Ser73) polyclonal Antibody detects Keratin 8 only when phosphorylated at Ser73. Validated for WB, IHC and ELISA. Reactive for Human, Mouse. AssayBiotechnology an original antibody manufacturer.
Details
  • Product Name
  • Catalog No
  • Storage/Stability
    Stable at -20°C for at least 1 year.
  • Applications
    WB, IHC, ELISA
  • Clonality
    Polyclonal
  • Host Species
    Rabbit
  • Reactivity
    Human, Mouse
  • Application Dilutions
    WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000
  • Database Links
    Gene ID: 3856, Swiss-Prot #: P05787, OMIM #: 148060/215600, Unigene #: Hs.533782/Hs.708445
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human Keratin 8 around the phosphorylation site of Ser73.
  • Immunogen Range
    41-90
  • Modification Sites
    H: S73 M: S79
  • MW
    53 kDa
  • NCBI Gene Symbol
    KRT8
  • Physical Form
    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Purification
    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
  • Specificity
    Keratin 8 (Phospho-Ser73) Antibody detects endogenous levels of Keratin 8 only when phosphorylated at Ser73.
  • Synonyms
    CK 8, CK8, CYK8, Cytokeratin endo A, K2C8, K8, KRT2-8, KRT8, Keratin, type II cytoskeletal 8, cytokeratin 8
  • Target Modification
    Phospho
Application Images
Image 1 Western blot analysis of lysates from 293 cells treated with Etoposide 25uM 60', using Keratin 8 (Phospho-Ser73) Antibody. The lane on the right is blocked with the phospho peptide.
Image 2 Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Keratin 8 (Phospho-Ser73) Antibody. The picture on the right is blocked with the phospho peptide.
Image 3 Western Blot analysis of various cells using Phospho-Cytokeratin 8 (S73) Antibody diluted at 1:500
Image 4 Western Blot analysis of 293 cells using Phospho-Cytokeratin 8 (S73) Antibody diluted at 1:500
Image 5 Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,ON). Negative control (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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