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Keratin 18 (Phospho-Ser33) Antibody

Catalog No : A0016
  • Applications
    WB , IHC , IF , ELISA
  • Reactivity
    Human , Mouse , Rat
  • Application Dilutions
    WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000
  • Size
    50ul
  • Price
    $195.00
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Anti-Keratin 18 (Phospho-Ser33) polyclonal Antibody detects Keratin 18 only when phosphorylated at Ser33. Validated for WB, IHC, IF and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
  • Product Name
  • Catalog No
  • Storage/Stability
    Stable at -20°C for at least 1 year.
  • Applications
    WB, IHC, IF, ELISA
  • Clonality
    Polyclonal
  • Host Species
    Rabbit
  • Reactivity
    Human, Mouse, Rat
  • Application Dilutions
    WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000
  • Database Links
    Gene ID: 3875, Swiss-Prot #: P05783, OMIM #: 148070/215600, Unigene #: Hs.406013
  • Image 1
    Immunofluorescence analysis of HeLa cells, using Keratin 18 (Phospho-Ser33) Antibody. The picture on the right is blocked with the phospho peptide.
  • Image 2
    Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Keratin 18 (Phospho-Ser33) Antibody. The picture on the right is blocked with the phospho peptide.
  • Image 3
    Western blot analysis of lysates from HT29 cells, using Keratin 18 (Phospho-Ser33) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human Keratin 18 around the phosphorylation site of Ser33.
  • Immunogen Range
    1-50
  • Modification Sites
    H: S34 M: S35 R: S35
  • MW
    47 kDa
  • NCBI Gene Symbol
    KRT18
  • Physical Form
    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Purification
    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
  • Specificity
    Keratin 18 (Phospho-Ser33) Antibody detects endogenous levels of Keratin 18 only when phosphorylated at Ser33.
  • Synonyms
    CK 18, CK18, CYK18, Cytokeratin endo B, K18, K1C18, KERD, KRT1-18, KRT18, Keratin, Keratin D, Keratin, type I cytoskeletal 18, cytokeratin 18, type I cytoskeletal 18
  • Target Modification
    Phospho
Application Images
Image 1 Western blot analysis of lysates from HT29 cells, using Keratin 18 (Phospho-Ser33) Antibody. The lane on the right is blocked with the phospho peptide.
Image 2 Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Keratin 18 (Phospho-Ser33) Antibody. The picture on the right is blocked with the phospho peptide.
Image 3 Immunofluorescence analysis of HeLa cells, using Keratin 18 (Phospho-Ser33) Antibody. The picture on the right is blocked with the phospho peptide.
Image 4 Western Blot analysis of various cells using Phospho-Cytokeratin 18 (S33) Antibody diluted at 1:2000
Image 5 Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,ON). Negative control (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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