The JNK1/2/3 (Phospho-Thr183+Tyr185) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect JNK1/2/3 protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated JNK1/2/3 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on JNK1/2/3 phosphorylation.
Details
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Product Name
JNK1/2/3 (Phospho-Thr183+Tyr185) Colorimetric Cell-Based ELISA Kit
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Catalog No
CBP1169
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Detection Method
Colorimetric 450 nm
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Dynamic Range
> 5000 Cells
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Storage/Stability
4°C/6 Months
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Reactivity
Human, Mouse, Rat
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Assay Type
Cell-Based
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Database Links
Gene ID: 5599/5601/5602, UniProt ID: P45983/P45984/P53779, OMIM #: 601158/602896/602897/606369, Unigene #: Hs.138211/Hs.654461/Hs.125503
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Format
2 x 96-Well Microplates
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Manual (PDF)
Download
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NCBI Gene Symbol
MAPK8/9/10
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Sub Type
Phospho
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Synonyms
JNK-46, JNK1-alpha-2, MAPK8, MK08, Mitogen-activated protein kinase 8, PRKM8, SAPK gamma, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1, kinase JNK1, p54 gamma
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TargetName
JNK1/2/3 (Phospho-Thr183+Tyr185)
Application Images
Western blot analysis of extracts from 293 cells treated with UV 5', using JNK1/2/3 (Phospho-Thr183+Tyr185) Antibody.
An illustrated breakdown of the colorimetric Non-Phospho Cell-Based ELISA assay principles.
A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits.
The Anti-GAPDH Antibody is a mouse monoclonal antibody used as a positive control in Assay Biotechnology's Cell-Based ELISA kits. It was tested on Western Blots with the tissue lysates from human, mouse, and rat for specificity.
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Western blot analysis of extracts from 293 cells treated with UV 5', using JNK1/2/3 (Phospho-Thr183+Tyr185) Antibody. |
|
An illustrated breakdown of the colorimetric Non-Phospho Cell-Based ELISA assay principles. |
|
A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits. |
|
The Anti-GAPDH Antibody is a mouse monoclonal antibody used as a positive control in Assay Biotechnology's Cell-Based ELISA kits. It was tested on Western Blots with the tissue lysates from human, mouse, and rat for specificity. |
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