Indirect ELISA - Protocol
ELISA (enzyme-linked immunoSorbent assay) is an immunoassay that detects antigens via antibody binding that
are linked to enzymes to allow for a diverse choice of detection methods. The indirect ELISA detects antigens
that have been immobilized onto solid surfaces, commonly in the form of microplates. Once non-specific sites
are blocked, primary antibodies and then enzyme conjugated secondary antibodies are incubated to build a
complex for detection. Detection methods can include colorimetric, fluorometric, or luminometric depending on
the type of secondary antibody conjugation.