Immunohistochemistry (IHC) Polymer - Protocol
Immunohistochemistry is an important application of immunestaining in histology. It entails the process of specifically
detecting antigens in cells by using the antibodies, which bind to these antigens in the biological tissues. Tissue
samples are fixed via paraffin-embedded or formalin-fixed methods and are pre-treated with an antigen retrieval agent.
Pre-treatment is essential to improve expression of the antigen by breaking formalin induced antigen cross linking.
Slides are then blocked to reduce background and can be stained via direct labelling or indirect assays.