Immunofluorescence (IF) - Protocol
Immunofluorescence is an immunostaining technique that visualizes antigens in samples using fluorescent microscopy.
Samples are fixed carefully with organic solvents and chemical crosslinkers to maintain their cellular integrity
as well as sub-cellular architecture. They are then permeabilized to allow immunostaining and then blocked to minimize
non-specific binding. Samples are then immunostained with antibodies and via fluorescent reaction targets are visualized