Anti-IkappaB-beta (Phospho-Ser23) polyclonal Antibody detects IkappaB-beta only when phosphorylated at Ser23. Validated for WB, IHC, IF and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
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Product Name
IkappaB-beta (Phospho-Ser23) Antibody
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Catalog No
A0014
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Storage/Stability
Stable at -20°C for at least 1 year.
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Applications
WB, IHC, IF, ELISA
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Clonality
Polyclonal
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Host Species
Rabbit
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Reactivity
Human, Mouse, Rat
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Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000
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Database Links
Gene ID: 4793, Swiss-Prot #: Q15653, OMIM #: 604495, Unigene #: Hs.9731
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Immunogen
The antiserum was produced against synthesized peptide derived from human IkappaB-beta around the phosphorylation site of Ser23.
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Immunogen Range
8-57
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Modification Sites
H: S23 M: S23 R: S23
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MW
37 kDa
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NCBI Gene Symbol
NFKBIB
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Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
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Specificity
IkappaB-beta (Phospho-Ser23) Antibody detects endogenous levels of IkappaB-beta only when phosphorylated at Ser23.
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Synonyms
I-kappa-B-beta, IKB-B, IKBB, IkappaBbeta, NF-kappa-BIB, NF-kappaB inhibitor beta, NFKBIB, TR- interacting protein 9, TRIP9, Thyroid receptor interacting protein 9
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Target Modification
Phospho
Application Images
Western blot analysis of lysates from HeLa cells treated with TNF-a 20ng/ml 5', using IkappaB-beta (Phospho-Ser23) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using IkappaB-beta (Phospho-Ser23) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of HeLa cells treated with TNF-a 20nM 15', using IkappaB-beta (Phospho-Ser23) Antibody. The picture on the right is blocked with the phospho peptide.
Western Blot analysis of various cells using Phospho-IkappaB-beta (S23) Antibody
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Western blot analysis of lysates from HeLa cells treated with TNF-a 20ng/ml 5', using IkappaB-beta (Phospho-Ser23) Antibody. The lane on the right is blocked with the phospho peptide. |
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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using IkappaB-beta (Phospho-Ser23) Antibody. The picture on the right is blocked with the phospho peptide. |
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Immunofluorescence analysis of HeLa cells treated with TNF-a 20nM 15', using IkappaB-beta (Phospho-Ser23) Antibody. The picture on the right is blocked with the phospho peptide. |
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Western Blot analysis of various cells using Phospho-IkappaB-beta (S23) Antibody |
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