Anti-ITK (Phospho-Tyr512) polyclonal Antibody detects ITK only when phosphorylated at Tyr512. Validated for IHC and ELISA. Reactive for Human, Mouse. AssayBiotechnology an original antibody manufacturer.
Details
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Product Name
ITK (Phospho-Tyr512) Antibody
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Catalog No
A1079
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Storage/Stability
Stable at -20°C for at least 1 year.
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Applications
WB, IHC, ELISA
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Clonality
Polyclonal
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Host Species
Rabbit
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Reactivity
Human, Mouse
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Application Dilutions
IHC: 1:50~1:100 ELISA: 1:10000
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Database Links
Gene ID: 3702, Swiss-Prot #: Q08881, OMIM #: 186973, Unigene #: Hs.558348
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Immunogen
The antiserum was produced against synthesized peptide derived from human ITK around the phosphorylation site of Tyr512.
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Immunogen Range
478-527
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Modification Sites
H: Y512, M: Y517
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MW
71 kDa
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NCBI Gene Symbol
ITK
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Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
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Specificity
ITK (Phospho-Tyr512) Antibody detects endogenous levels of ITK only when phosphorylated at Tyr512.
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Synonyms
EMT, IL-2-inducible T-cell kinase, IL2-inducible T-cell kinase, ITK/TSK, Kinase EMT, kinase Itk, LYK, Lyk, T-cell-specific kinase, TLK, TSK
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Target Modification
Phospho
Application Images
Western blot analysis of 293 using p-Emt (Y512) antibody.
Immunohistochemical analysis of paraffin-embedded Human testis. Antibody was diluted at 1:100(4°,ON). Negative control (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Western blot analysis of 293 using p-Emt (Y512) antibody. |
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Immunohistochemical analysis of paraffin-embedded Human testis. Antibody was diluted at 1:100(4°,ON). Negative control (right) obtaned from antibody was pre-absorbed by immunogen peptide. |
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