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IRS-1 (Phospho-Ser612) Antibody

Catalog No : A0447
  • Applications
    WB, IHC, ELISA
  • Reactivity
    Human, Mouse, Rat
  • Size
    50ul
  • Price
    $195.00
Anti-IRS-1 (Phospho-Ser612) polyclonal Antibody detects IRS-1 only when phosphorylated at Ser612. Validated for WB, IHC and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
  • Product Name
    IRS-1 (Phospho-Ser612) Antibody
  • Catalog No
    A0447
  • Storage/Stability
    Stable at -20°C for at least 1 year.
  • Applications
    WB, IHC, ELISA
  • Clonality
    Polyclonal
  • Host Species
    Rabbit
  • Reactivity
    Human, Mouse, Rat
  • Application Dilutions
    WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000
  • Database Links
    Gene ID: 3667, Swiss-Prot #: P35568, OMIM #: 125853/147545, Unigene #: Hs.471508
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human IRS-1 around the phosphorylation site of Ser612.
  • Immunogen Range
    578-627
  • Modification Sites
    H: S616 M: S612 R: S612
  • MW
    131 kDa
  • NCBI Gene Symbol
    IRS1
  • Physical Form
    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Purification
    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
  • Specificity
    IRS-1 (Phospho-Ser612) Antibody detects endogenous levels of IRS-1 only when phosphorylated at Ser612.
  • Synonyms
    Insulin receptor substrate-1, IRS-1, IRS1
  • Target Modification
    Phospho
Application Images
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IRS-1 (Phospho-Ser612) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from HUVEC cells treated with insulin 0.01U/ml 30', using IRS-1 (Phospho-Ser612) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IRS-1 (Phospho-Ser612) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from HUVEC cells treated with insulin 0.01U/ml 30', using IRS-1 (Phospho-Ser612) Antibody. The lane on the right is blocked with the phospho peptide.