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IRS-1 (Phospho-Ser307) Fluorometric Cell-Based ELISA Kit

Catalog No : FLUO-CBP1090
  • Reactivity
    Human , Mouse , Rat
  • Assay Type
    Cell-Based ELISA
  • Size
    1 Kit
  • Price
    $480.00
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The IRS-1 (Phospho-Ser307) Fluorometric Cell-Based Phospho ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor IRS-1 (Phospho-Ser307) protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated IRS-1 (Phospho-Ser307) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals, or activators have on IRS1 phosphorylation.
Details
  • Product Name
  • Catalog No
  • Detection Method
    Fluorometric
  • Dynamic Range
    > 5000 Cells
  • Storage/Stability
    4°C/6 Months
  • Reactivity
    Human, Mouse, Rat
  • Assay Type
    Cell-Based ELISA
  • Database Links
    Gene ID: 3667, UniProt ID: P35568, OMIM #: 125853/147545, Unigene #: Hs.471508
  • Format
    Two 96-Well Plates
  • Manual (PDF)
  • NCBI Gene Symbol
    IRS1
  • Sub Type
    Phospho
  • Synonyms
    Insulin receptor substrate-1, IRS-1, IRS1
  • TargetName
    Phospho-IRS-1 (Ser307)
Application Images
Image 1 Western blot analysis of extracts from K562 cells, using IRS-1 (Phospho-Ser307) Antibody.
Image 2 Hek 293 cells were starved for 24 hrs. and stimulated with PMA 200nM for 2 hrs.
Image 3 An illustrated breakdown of the colorimetric Non-Phospho Cell-Based ELISA assay principles.
Image 4 A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits.
Image 5 The Anti-GAPDH Antibody is a mouse monoclonal antibody used as a positive control in Assay Biotechnology's Cell-Based ELISA kits. It was tested on Western Blots with the tissue lysates from human, mouse, and rat for specificity.
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