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IKK-alpha (Phospho-Ser176) /IKK-beta (Phospho-Ser177) Antibody

Catalog No : A0441
  • Applications
    WB , IHC , ELISA
  • Reactivity
    Human , Mouse , Rat
  • Application Dilutions
    WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
  • Size
    50ul
  • Price
    $195.00
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Anti-IKK-alpha (Phospho-Ser176) /IKK-beta (Phospho-Ser177) polyclonal Antibody detects IKK-alpha only when phosphorylated at Ser177. Validated for WB, IHC and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
  • Product Name
  • Catalog No
  • Storage/Stability
    Stable at -20°C for at least 1 year.
  • Applications
    WB, IHC, ELISA
  • Clonality
    Polyclonal
  • Host Species
    Rabbit
  • Reactivity
    Human, Mouse, Rat
  • Application Dilutions
    WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
  • Database Links
    Gene ID: 1147/3551, Swiss-Prot #: O15111/O14920, OMIM #: 600664/603258, Unigene #: Hs.198998/Hs.656458
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human IKK-alpha around the phosphorylation site of Ser177.
  • Immunogen Range
    151-200
  • Modification Sites
    H: S176/S177 M: S176/S177 R: S176/S177
  • MW
    84 kDa
  • NCBI Gene Symbol
    CHUK
  • Physical Form
    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Purification
    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
  • Specificity
    IKK-alpha (Phospho-Ser176) /IKK-beta (Phospho-Ser177) Antibody detects endogenous levels of IKK-alpha only when phosphorylated at Ser177.
  • Synonyms
    CHUK, Conserved helix-loop-helix ubiquitous kinase, I kappa-B kinase alpha, I-kappa-B kinase 1, IkappaB kinase, IkBKA, IKK-A, IKK-alpha, IKK1, IKKA, Inhibitor of nuclear factor kappa-B kinase alpha subunit, kinase IKK-alpha, NFKBIKA, Nuclear factor NFkapp
  • Target Modification
    Phospho
Application Images
Image 1 Western blot analysis of lysates from NIH/3T3 cells treated with TNF 20ng/ml 30', using IKK-alpha (Phospho-Ser176) /IKK-beta (Phospho-Ser177) Antibody. The lane on the right is blocked with the phospho peptide.
Image 2 Immunohistochemistry analysis of paraffin-embedded human heart, using IKK-alpha (Phospho-Ser176) /IKK-beta (Phospho-Ser177) Antibody. The picture on the right is blocked with the phospho peptide.
Image 3 Western Blot analysis of NIH-3T3 cells using Phospho-IKKalpha/beta (S176/177) Antibody diluted at 1:1000
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