The HSP90B (Phospho-Ser254) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect HSP90B protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated HSP90B in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on HSP90B phosphorylation.
Details
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Product Name
HSP90B (Phospho-Ser254) Colorimetric Cell-Based ELISA Kit
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Catalog No
CBP1013
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Detection Method
Colorimetric 450 nm
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Dynamic Range
> 5000 Cells
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Storage/Stability
4°C/6 Months
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Reactivity
Human, Mouse, Rat
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Assay Type
Cell-Based
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Database Links
Gene ID: 3326, UniProt ID: P08238, OMIM #: 140572, Unigene #: Hs.509736/Hs.689683
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Format
2 x 96-Well Microplates
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Manual (PDF)
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NCBI Gene Symbol
HSP90AB1
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Sub Type
Phospho
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Synonyms
HS90B, HSP 84, HSP90-beta, HSP90AB1, HSPC2, HSPCB, Heat shock protein HSP 90-beta
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TargetName
HSP90B (Phospho-Ser254)
Application Images
Western blot analysis of extracts from HeLa cells treated with TNF-a 10ng/ml 30', using HSP90B (Phospho-Ser254) Antibody.
An illustrated breakdown of the colorimetric Non-Phospho Cell-Based ELISA assay principles.
A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits.
The Anti-GAPDH Antibody is a mouse monoclonal antibody used as a positive control in Assay Biotechnology's Cell-Based ELISA kits. It was tested on Western Blots with the tissue lysates from human, mouse, and rat for specificity.
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Western blot analysis of extracts from HeLa cells treated with TNF-a 10ng/ml 30', using HSP90B (Phospho-Ser254) Antibody. |
|
An illustrated breakdown of the colorimetric Non-Phospho Cell-Based ELISA assay principles. |
|
A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits. |
|
The Anti-GAPDH Antibody is a mouse monoclonal antibody used as a positive control in Assay Biotechnology's Cell-Based ELISA kits. It was tested on Western Blots with the tissue lysates from human, mouse, and rat for specificity. |
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