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HSP90B (Phospho-Ser254) Antibody

Catalog No : A0013
  • Applications
    WB , IHC , IF , ELISA
  • Reactivity
    Human , Mouse , Rat
  • Application Dilutions
    WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000
  • Size
    50ul
  • Price
    $195.00
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Anti-HSP90B (Phospho-Ser254) polyclonal Antibody detects HSP90B only when phosphorylated at Ser254. Validated for WB, IHC, IF and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
  • Product Name
  • Catalog No
  • Storage/Stability
    Stable at -20°C for at least 1 year.
  • Applications
    WB, IHC, IF, ELISA
  • Clonality
    Polyclonal
  • Host Species
    Rabbit
  • Reactivity
    Human, Mouse, Rat
  • Application Dilutions
    WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000
  • Database Links
    Gene ID: 3326, Swiss-Prot #: P08238, OMIM #: 140572, Unigene #: Hs.509736/Hs.689683
  • Image 2
    Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HSP90B (Phospho-Ser254) Antibody. The picture on the right is blocked with the phospho peptide.
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human HSP90B around the phosphorylation site of Ser254.
  • Immunogen Range
    226-275
  • Modification Sites
    H: S254 M: S254 R: S254
  • MW
    83 kDa
  • NCBI Gene Symbol
    HSP90AB1
  • Physical Form
    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Purification
    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
  • Specificity
    HSP90B (Phospho-Ser254) Antibody detects endogenous levels of HSP90B only when phosphorylated at Ser254.
  • Synonyms
    HS90B, HSP 84, HSP90-beta, HSP90AB1, HSPC2, HSPCB, Heat shock protein HSP 90-beta
  • Target Modification
    Phospho
Application Images
Image 1 Western blot analysis of lysates from HeLa cells treated with TNF-a 10ng/ml 30', using HSP90B (Phospho-Ser254) Antibody. The lane on the right is blocked with the phospho peptide.
Image 2 Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HSP90B (Phospho-Ser254) Antibody. The picture on the right is blocked with the phospho peptide.
Image 3 Immunofluorescence analysis of HeLa cells treated with TNF-a 20nM 15', using HSP90B (Phospho-Ser254) Antibody. The picture on the right is blocked with the phospho peptide.
Image 4 Western Blot analysis of various cells using Phospho-HSP90beta (S254) Antibody
Image 5 Western Blot analysis of HepG2 cells using Phospho-HSP90beta (S254) Antibody
Image 6 Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,ON). Negative control (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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