Anti-HSP90B (Phospho-Ser254) Antibody (A0013) Rabbit Polyclonal

Description

Anti-HSP90B (Phospho-Ser254) polyclonal Antibody detects HSP90B only when phosphorylated at Ser254. Validated for WB, IHC, IF and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.

Details

Product Name

HSP90B (Phospho-Ser254) Antibody
Catalog No

A0013
Storage/Stability

Stable at -20°C for at least 1 year.
Applications

WB, IHC, IF, ELISA
Clonality

Polyclonal
Host Species

Rabbit
Reactivity

Human, Mouse, Rat
Application Dilutions

WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000
Database Links

Gene ID: 3326, Swiss-Prot #: P08238, OMIM #: 140572, Unigene #: Hs.509736/Hs.689683
Immunogen

The antiserum was produced against synthesized peptide derived from human HSP90B around the phosphorylation site of Ser254.
Immunogen Range

226-275
Modification Sites

H: S254 M: S254 R: S254
MW

83 kDa
NCBI Gene Symbol

HSP90AB1
Physical Form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification

The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Specificity

HSP90B (Phospho-Ser254) Antibody detects endogenous levels of HSP90B only when phosphorylated at Ser254.
Synonyms

HS90B, HSP 84, HSP90-beta, HSP90AB1, HSPC2, HSPCB, Heat shock protein HSP 90-beta
Target Modification

Phospho

Application Images

	Western blot analysis of lysates from HeLa cells treated with TNF-a 10ng/ml 30', using HSP90B (Phospho-Ser254) Antibody. The lane on the right is blocked with the phospho peptide.
	Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HSP90B (Phospho-Ser254) Antibody. The picture on the right is blocked with the phospho peptide.
	Immunofluorescence analysis of HeLa cells treated with TNF-a 20nM 15', using HSP90B (Phospho-Ser254) Antibody. The picture on the right is blocked with the phospho peptide.
	Western Blot analysis of various cells using Phospho-HSP90beta (S254) Antibody
	Western Blot analysis of HepG2 cells using Phospho-HSP90beta (S254) Antibody
	Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,ON). Negative control (right) obtaned from antibody was pre-absorbed by immunogen peptide.

By Product Type

By Application

IHC：(Immunohistochemistry)

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