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HSL (Phospho-Ser855/554) Antibody

Catalog No : A0074
  • Applications
    WB, IHC, ELISA
  • Reactivity
    Human, Mouse, Rat
  • Size
    50ul
  • Price
    $195.00
Anti-HSL (Phospho-Ser855/554) polyclonal Antibody detects HSL only when phosphorylated at Ser855/554. Validated for WB, IHC and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
  • Product Name
    HSL (Phospho-Ser855/554) Antibody
  • Catalog No
    A0074
  • Storage/Stability
    Stable at -20°C for at least 1 year.
  • Applications
    WB, IHC, ELISA
  • Clonality
    Polyclonal
  • Host Species
    Rabbit
  • Reactivity
    Human, Mouse, Rat
  • Application Dilutions
    WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
  • Database Links
    Gene ID: 3991, Swiss-Prot #: Q05469, OMIM #: 151750, Unigene #: Hs.656980
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human HSL around the phosphorylation site of Ser855/554.
  • Immunogen Range
    520-569
  • Modification Sites
    H: S855 M: S559 R: S865
  • MW
    116 kDa
  • NCBI Gene Symbol
    LIPE
  • Physical Form
    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Purification
    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
  • Specificity
    HSL (Phospho-Ser855/554) Antibody detects endogenous levels of HSL only when phosphorylated at Ser855/554.
  • Synonyms
    Hormone sensitive lipase, LIPE, LIPS
  • Target Modification
    Phospho
Application Images
Western blot analysis of lysates from HeLa cells treated with Adriamycin 0.5ng/ml 24h, using HSL (Phospho-Ser855/554) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using HSL (Phospho-Ser855/554) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of L929 using p-HSL (S855) antibody. Antibody was diluted at 1:1000
Western blot analysis of lysates from HeLa cells treated with Adriamycin 0.5ng/ml 24h, using HSL (Phospho-Ser855/554) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using HSL (Phospho-Ser855/554) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of L929 using p-HSL (S855) antibody. Antibody was diluted at 1:1000