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HER2 (Phospho-Tyr1221/Tyr1222) Antibody

Catalog No : A7103
  • Applications
    WB , IHC , IP , ELISA
  • Reactivity
    Human , Mouse , Rat
  • Application Dilutions
    WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
  • Size
    50ul
  • Price
    $195.00
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Anti-HER2 (Phospho-Tyr1221/Tyr1222) polyclonal Antibody detects HER2 only when phosphorylated at Tyr1221/Tyr1222. Validated for WB IHC IP ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
  • Product Name
  • Catalog No
  • Storage/Stability
    Stable at -20°C for at least 1 year.
  • Applications
    WB, IHC, IP, ELISA
  • Clonality
    Polyclonal
  • Host Species
    Rabbit
  • Reactivity
    Human, Mouse, Rat
  • Application Dilutions
    WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
  • Database Links
    Gene ID: 2064, Swiss-Prot #: P04626, OMIM #: 164870, Unigene #: Hs.446352
  • Image 1
    Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HER2 (Phospho-Tyr1221/Tyr1222) Antibody. The picture on the right is blocked with the phospho peptide.
  • Image 2
    Western blot analysis of lysates from SK-OV3 cells treated with EGF, using HER2 (Phospho-Tyr1221/Tyr1222) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human HER2 around the phosphorylation site of Tyr1221/Tyr1222.
  • Immunogen Range
    1191-1240
  • Modification Sites
    H: Y1221+Y1222 M: Y1222+Y1223 R: Y1223+Y1224
  • MW
    137 kDa
  • NCBI Gene Symbol
    ERBB2
  • Physical Form
    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Purification
    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
  • Specificity
    HER2 (Phospho-Tyr1221/Tyr1222) Antibody detects endogenous levels of HER2 only when phosphorylated at Tyr1221/Tyr1222.
  • Synonyms
    C-erbB-2, Epidermal growth factor receptor-related protein, ErbB2, HER2, kinase ErbB2, MLN 19, NEU, NEU proto-oncogene, NGL, p185erbB2, Receptor protein-tyrosine kinase erbB-2, Receptor protein-tyrosine kinase erbB-2 precursor, Tyrosine kinase-type cell s
  • Target Modification
    Phospho
Application Images
Image 1 Western blot analysis of lysates from SK-OV3 cells treated with EGF, using HER2 (Phospho-Tyr1221/Tyr1222) Antibody. The lane on the right is blocked with the phospho peptide.
Image 2 Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HER2 (Phospho-Tyr1221/Tyr1222) Antibody. The picture on the right is blocked with the phospho peptide.
Image 3 Western Blot analysis of various cells using Phospho-Neu (Y1221/Y1222) Antibody
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