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HDAC Activity Assay Kit - Fluorometric

Catalog No : EZ-0517
  • Size
    200 Assays
  • Price
    $195.00
This assay conveniently detects HDAC activity in cell lysates and solutions, or screens for HDAC inhibitor in cell extracts and purified enzymes. It uses a non-peptide HDAC substrate that, in comparison to peptide-based HDAC substrate, demonstrates more resistance to protease hydrolysis as well as higher sensitivity. This non-peptide substrate is cleaved into a highly fluorescent green product with long wavelength emission, allowing for less interference with cell components and other compounds. Signals increase with greater HDAC activity, and can be read with a fluorescence microplate reader with excitation at 490 nm and emission at 525 nm.
Details
  • Product Name
    HDAC Activity Assay Kit - Fluorometric
  • Catalog No
    EZ-0517
  • Detection Method
    Fluorometric
  • Storage/Stability
    -20°, C/3 Months
  • Fluorometric Readout [Ex (nm), Em (nm)]
    490, 525
  • Manual
    Download
  • Tar Name
    HDAC Activity
Application Images
Image 1 The HDAC Activity Assay Kit - Fluorometric allows for the detection and quantification of endogenous HDAC Activity in cell lysates, sera, and plasma.
Image 2