| This assay conveniently detects HDAC activity in cell lysates and solutions, or screens for HDAC inhibitor in cell extracts and purified enzymes. It uses a non-peptide HDAC substrate that, in comparison to peptide-based HDAC substrate, demonstrates more resistance to protease hydrolysis as well as higher sensitivity. This non-peptide substrate is cleaved into a highly fluorescent green product with long wavelength emission, allowing for less interference with cell components and other compounds. Signals increase with greater HDAC activity, and can be read with a fluorescence microplate reader with excitation at 490 nm and emission at 525 nm. |
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The HDAC Activity Assay Kit - Fluorometric allows for the detection and quantification of endogenous HDAC Activity in cell lysates, sera, and plasma. |
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EZ-0501
EZ-0502
EZ-0503
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