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GSK3 beta (Phospho-Ser9) Antibody

Catalog No : A7098
  • Applications
    WB , IHC , IP , ELISA
  • Reactivity
    Human , Mouse , Rat
  • Application Dilutions
    WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000
  • Size
    50ul
  • Price
    $195.00
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Anti-GSK3 beta (Phospho-Ser9) polyclonal Antibody detects GSK3 beta only when phosphorylated at Ser9. Validated for WB IHC IP ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
  • Product Name
  • Catalog No
  • Storage/Stability
    Stable at -20°C for at least 1 year.
  • Applications
    WB, IHC, IP, ELISA
  • Clonality
    Polyclonal
  • Host Species
    Rabbit
  • Reactivity
    Human, Mouse, Rat
  • Application Dilutions
    WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000
  • Database Links
    Gene ID: 2932, Swiss-Prot #: P49841, OMIM #: 605004, Unigene #: Hs.445734
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human GSK3 beta around the phosphorylation site of Ser9.
  • Immunogen Range
    1-50
  • Modification Sites
    Human, Mouse, Rat
  • MW
    46 kDa
  • NCBI Gene Symbol
    GSK3B
  • Physical Form
    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Purification
    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
  • Specificity
    GSK3 beta (Phospho-Ser9) Antibody detects endogenous levels of GSK3 beta only when phosphorylated at Ser9.
  • Synonyms
    Factor A, GSK-3 alpha, GSK3A, Glycogen synthase kinase-3 alpha, kinase GSK3-alpha
  • Target Modification
    Phospho
Application Images
Image 1 Western blot analysis of lysates from HeLa cells treated with EGF, using GSK3 beta (Phospho-Ser9) Antibody. The lane on the right is blocked with the phospho peptide.
Image 2 Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using GSK3 beta (Phospho-Ser9) Antibody. The picture on the right is blocked with the phospho peptide.
Image 3 Western Blot analysis of various cells using Phospho-GSK3beta (S9) Antibody diluted at 1:1000
Image 4 Western Blot analysis of Hela cells using Phospho-GSK3beta (S9) Antibody diluted at 1:1000
Image 5 Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. GSK3beta (phospho Ser9) Antibody was diluted at 1:200(4°C,ON). Negative control was defined by secondary antibody only.
Image 6 Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. GSK3beta (phospho Ser9) Antibody was diluted at 1:200(4°C,ON). Negative control was defined by secondary antibody only.
Image 7 Immunofluorescence analysis of rat-spleen tissue. GSK3beta (phospho Ser9) Antibody(red) was diluted at 1:200(4°C,ON). Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Image 8 Immunofluorescence analysis of rat-spleen tissue. GSK3beta (phospho Ser9) Antibody(red) was diluted at 1:200(4°C,ON). Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
References
This product has been referenced in:
  • Ling-Zhi Xu,1 Zi-Jie Long,2,3 Fei Peng,1 Yang Liu,1 Jie Xu,1 Chang Wang,1 Lei Jiang,1 Tao Guo,1 Muha Aurora kinase A suppresses metabolic stress-induced autophagic cell death by activating mTOR signaling in breast cancer cells NCBI PMC Primary Antibody "doi: 10.18632/oncotarget.2241 PMCID: PMC4202139 PMID: 25115395" Read more >
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