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> Products > Antibodies > Modification-Specific Antibodies

GSK3 alpha/beta (Phospho-Tyr279/216) Antibody

Datasheet SDS
Catalog No : A0012
  • Applications
    WB , IHC , ELISA
  • Reactivity
    Human , Mouse , Rat
  • Application Dilutions
    WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
  • Size
    50ul
  • Price
    $195.00
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Anti-GSK3 alpha/beta (Phospho-Tyr279/216) polyclonal Antibody detects GSK3 alpha/beta only when phosphorylated at Tyr279/216. Validated for WB, IHC and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
  • Product Name
    GSK3 alpha/beta (Phospho-Tyr279/216) Antibody
  • Catalog No
    A0012
  • Storage/Stability
    Stable at -20°C for at least 1 year.
  • Applications
    WB, IHC, ELISA
  • Clonality
    Polyclonal
  • Host Species
    Rabbit
  • Reactivity
    Human, Mouse, Rat
  • Application Dilutions
    WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
  • Database Links
    Gene ID: 2931, Swiss-Prot #: P49840, OMIM #: 606784, Unigene #: Hs.466828
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human GSK3 alpha/beta around the phosphorylation site of Tyr279/216.
  • Immunogen Range
    246-295
  • Modification Sites
    H: Y279 M: Y279 R: Y279
  • MW
    50 kDa
  • NCBI Gene Symbol
    GSK3A
  • Physical Form
    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Purification
    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
  • Specificity
    GSK3 alpha/beta (Phospho-Tyr279/216) Antibody detects endogenous levels of GSK3 alpha/beta only when phosphorylated at Tyr279/216.
  • Synonyms
    Factor A, GSK-3 alpha, GSK3-alpha, Glycogen synthase kinase-3 alpha, kinase GSK3-alpha
  • Target Modification
    Phospho
Application Images
Image 1 Western blot analysis of lysates from HeLa cells, using GSK3 alpha/beta (Phospho-Tyr279/216) Antibody. The lane on the right is blocked with the phospho peptide.
Image 2 Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using GSK3 alpha/beta (Phospho-Tyr279/216) Antibody. The picture on the right is blocked with the phospho peptide.
Image 3 Western Blot analysis of various cells using primary antibody diluted at 1:1000(4°C ON). Secondary antibody?Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour).
Image 4 Western Blot analysis of various cells using Phospho-GSK3alpha/beta (Y279/216) Antibody
Image 5 Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. GSK3alpha/beta (phospho Tyr279/216) Antibody was diluted at 1:200(4°C,ON). Negative control was defined by secondary antibody only.
Image 6 Immunohistochemical analysis of paraffin-embedded Human-liver tissue. GSK3alpha/beta (phospho Tyr279/216) Antibody was diluted at 1:200(4°C,ON). Negative control was defined by secondary antibody only.
Image 7 Immunofluorescence analysis of mouse-liver tissue. GSK3alpha/beta (phospho Tyr279/216) Antibody(red) was diluted at 1:200(4°C,ON). Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Image 8 Immunofluorescence analysis of mouse-liver tissue. GSK3alpha/beta (phospho Tyr279/216) Antibody(red) was diluted at 1:200(4°C,ON). Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
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