Anti-GSK3 alpha/beta (Phospho-Tyr279/216) polyclonal Antibody detects GSK3 alpha/beta only when phosphorylated at Tyr279/216. Validated for WB, IHC and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
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Product Name
GSK3 alpha/beta (Phospho-Tyr279/216) Antibody
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Catalog No
A0012
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Storage/Stability
Stable at -20°C for at least 1 year.
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Applications
WB, IHC, ELISA
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Clonality
Polyclonal
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Host Species
Rabbit
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Reactivity
Human, Mouse, Rat
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Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
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Database Links
Gene ID: 2931, Swiss-Prot #: P49840, OMIM #: 606784, Unigene #: Hs.466828
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Immunogen
The antiserum was produced against synthesized peptide derived from human GSK3 alpha/beta around the phosphorylation site of Tyr279/216.
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Immunogen Range
246-295
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Modification Sites
H: Y279 M: Y279 R: Y279
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MW
50 kDa
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NCBI Gene Symbol
GSK3A
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Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
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Specificity
GSK3 alpha/beta (Phospho-Tyr279/216) Antibody detects endogenous levels of GSK3 alpha/beta only when phosphorylated at Tyr279/216.
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Synonyms
Factor A, GSK-3 alpha, GSK3-alpha, Glycogen synthase kinase-3 alpha, kinase GSK3-alpha
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Target Modification
Phospho
Application Images
Western blot analysis of lysates from HeLa cells, using GSK3 alpha/beta (Phospho-Tyr279/216) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using GSK3 alpha/beta (Phospho-Tyr279/216) Antibody. The picture on the right is blocked with the phospho peptide.
Western Blot analysis of various cells using primary antibody diluted at 1:1000(4°C ON). Secondary antibody?Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour).
Western Blot analysis of various cells using Phospho-GSK3alpha/beta (Y279/216) Antibody
Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. GSK3alpha/beta (phospho Tyr279/216) Antibody was diluted at 1:200(4°C,ON). Negative control was defined by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-liver tissue. GSK3alpha/beta (phospho Tyr279/216) Antibody was diluted at 1:200(4°C,ON). Negative control was defined by secondary antibody only.
Immunofluorescence analysis of mouse-liver tissue. GSK3alpha/beta (phospho Tyr279/216) Antibody(red) was diluted at 1:200(4°C,ON). Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of mouse-liver tissue. GSK3alpha/beta (phospho Tyr279/216) Antibody(red) was diluted at 1:200(4°C,ON). Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
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Western blot analysis of lysates from HeLa cells, using GSK3 alpha/beta (Phospho-Tyr279/216) Antibody. The lane on the right is blocked with the phospho peptide. |
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using GSK3 alpha/beta (Phospho-Tyr279/216) Antibody. The picture on the right is blocked with the phospho peptide. |
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Western Blot analysis of various cells using primary antibody diluted at 1:1000(4°C ON). Secondary antibody?Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour). |
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Western Blot analysis of various cells using Phospho-GSK3alpha/beta (Y279/216) Antibody |
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Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. GSK3alpha/beta (phospho Tyr279/216) Antibody was diluted at 1:200(4°C,ON). Negative control was defined by secondary antibody only. |
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Immunohistochemical analysis of paraffin-embedded Human-liver tissue. GSK3alpha/beta (phospho Tyr279/216) Antibody was diluted at 1:200(4°C,ON). Negative control was defined by secondary antibody only. |
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Immunofluorescence analysis of mouse-liver tissue. GSK3alpha/beta (phospho Tyr279/216) Antibody(red) was diluted at 1:200(4°C,ON). Picture A:Target. Picture B: DAPI. Picture C: merge of A+B |
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Immunofluorescence analysis of mouse-liver tissue. GSK3alpha/beta (phospho Tyr279/216) Antibody(red) was diluted at 1:200(4°C,ON). Picture A:Target. Picture B: DAPI. Picture C: merge of A+B |