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GRB10 (Phospho-Tyr67) Antibody

Catalog No : A1031
  • Applications
    WB, IF, ELISA
  • Reactivity
    Human
  • Size
    50ul
  • Price
    $195.00
Anti-GRB10 (Phospho-Tyr67) polyclonal Antibody detects GRB10 only when phosphorylated at Tyr67. Validated for WB, IF and ELISA. Reactive for Human. AssayBiotechnology an original antibody manufacturer.
Details
  • Product Name
    GRB10 (Phospho-Tyr67) Antibody
  • Catalog No
    A1031
  • Storage/Stability
    Stable at -20°C for at least 1 year.
  • Applications
    WB, IF, ELISA
  • Clonality
    Polyclonal
  • Host Species
    Rabbit
  • Reactivity
    Human
  • Application Dilutions
    WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000
  • Database Links
    Gene ID: 2887, Swiss-Prot #: Q13322, OMIM #: 601523, Unigene #: Hs.164060
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human GRB10 around the phosphorylation site of Tyr67.
  • Immunogen Range
    33-82
  • Modification Sites
    H: Y67
  • MW
    67 kDa
  • NCBI Gene Symbol
    GRB10
  • Physical Form
    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Purification
    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
  • Specificity
    GRB10 (Phospho-Tyr67) Antibody detects endogenous levels of GRB10 only when phosphorylated at Tyr67.
  • Synonyms
    GRB10 adaptor protein, GRBIR, Growth factor receptor-bound protein 10, Insulin receptor binding protein GRB-IR, KIAA0207
  • Target Modification
    Phospho
Application Images
Western blot analysis of lysates from NIH/3T3 cells treated with Insulin 0.01U/ml 15', using GRB10 (Phospho-Tyr67) Antibody. The lane on the right is blocked with the phospho peptide.
Immunofluorescence analysis of HepG2 cells, using GRB10 (Phospho-Tyr67) Antibody. The picture on the right is blocked with the phospho peptide.
Western Blot analysis of COLO cells using Phospho-GRB10 (Y67) Antibody
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4°,ON). Negative control (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Western blot analysis of lysates from NIH/3T3 cells treated with Insulin 0.01U/ml 15', using GRB10 (Phospho-Tyr67) Antibody. The lane on the right is blocked with the phospho peptide.
Immunofluorescence analysis of HepG2 cells, using GRB10 (Phospho-Tyr67) Antibody. The picture on the right is blocked with the phospho peptide.
Western Blot analysis of COLO cells using Phospho-GRB10 (Y67) Antibody
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4°,ON). Negative control (right) obtaned from antibody was pre-absorbed by immunogen peptide.