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FKHRL1 (Phospho-Ser253) Fluorometric Cell-Based ELISA Kit

Catalog No : FLUO-CBP1558
  • Reactivity
    Human, Mouse, Rat
  • Size
    1 Kit
  • Price
    $480.00
The FKHRL1 (Phospho-Ser253) Fluorometric Cell-Based Phospho ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor FKHRL1 (Phospho-Ser253) protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated FKHRL1 (Phospho-Ser253) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals, or activators have on FOXO3 phosphorylation.
Details
  • Product Name
    FKHRL1 (Phospho-Ser253) Fluorometric Cell-Based ELISA Kit
  • Catalog No
    FLUO-CBP1558
  • Detection Method
    Fluorometric
  • Dynamic Range
    > 5000 Cells
  • Storage/Stability
    4°C/6 Months
  • Reactivity
    Human, Mouse, Rat
  • Assay Type
    Cell-Based ELISA
  • Database Links
    Gene ID: 2309, UniProt ID: O43524, OMIM #: 602681, Unigene #: Hs.220950
  • Format
    Two 96-Well Plates
  • Manual (PDF)
  • NCBI Gene Symbol
    FOXO3
  • Sub Type
    Phospho
  • Synonyms
    AF6q21 protein, FKHR2, FKHRL1, Forkhead box O3, forkhead box O3A, Forkhead box protein O3A, Forkhead in rhabdomyosarcoma-like 1, FOXO3
  • TargetName
    Phospho-FKHRL1 (Ser253)
Application Images
Western blot analysis of extracts from LOVO cells treated with serum 20% 30', using FKHRL1 (Phospho-Ser253) Antibody.
Jurkat cells were starved for 24 hrs. and stimulated with 200nM PMA for 1 hr.
An illustrated breakdown of the colorimetric Non-Phospho Cell-Based ELISA assay principles.
A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits.
The Anti-GAPDH Antibody is a mouse monoclonal antibody used as a positive control in Assay Biotechnology's Cell-Based ELISA kits. It was tested on Western Blots with the tissue lysates from human, mouse, and rat for specificity.
Western blot analysis of extracts from LOVO cells treated with serum 20% 30', using FKHRL1 (Phospho-Ser253) Antibody.
Jurkat cells were starved for 24 hrs. and stimulated with 200nM PMA for 1 hr.
An illustrated breakdown of the colorimetric Non-Phospho Cell-Based ELISA assay principles.
A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits.
The Anti-GAPDH Antibody is a mouse monoclonal antibody used as a positive control in Assay Biotechnology's Cell-Based ELISA kits. It was tested on Western Blots with the tissue lysates from human, mouse, and rat for specificity.