FKHRL1 (Phospho-Ser253) Fluorometric Cell-Based ELISA Kit

Description

The FKHRL1 (Phospho-Ser253) Fluorometric Cell-Based Phospho ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor FKHRL1 (Phospho-Ser253) protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated FKHRL1 (Phospho-Ser253) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals, or activators have on FOXO3 phosphorylation.

Details

Product Name

FKHRL1 (Phospho-Ser253) Fluorometric Cell-Based ELISA Kit
Catalog No

FLUO-CBP1558
Detection Method

Fluorometric
Dynamic Range

> 5000 Cells
Storage/Stability

4°C/6 Months
Reactivity

Human, Mouse, Rat
Assay Type

Cell-Based ELISA
Database Links

Gene ID: 2309, UniProt ID: O43524, OMIM #: 602681, Unigene #: Hs.220950
Format

Two 96-Well Plates
Manual (PDF)

Download
NCBI Gene Symbol

FOXO3
Sub Type

Phospho
Synonyms

AF6q21 protein, FKHR2, FKHRL1, Forkhead box O3, forkhead box O3A, Forkhead box protein O3A, Forkhead in rhabdomyosarcoma-like 1, FOXO3
TargetName

Phospho-FKHRL1 (Ser253)

Application Images

Western blot analysis of extracts from LOVO cells treated with serum 20% 30', using FKHRL1 (Phospho-Ser253) Antibody.

Jurkat cells were starved for 24 hrs. and stimulated with 200nM PMA for 1 hr.

An illustrated breakdown of the colorimetric Non-Phospho Cell-Based ELISA assay principles.

A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits.

The Anti-GAPDH Antibody is a mouse monoclonal antibody used as a positive control in Assay Biotechnology's Cell-Based ELISA kits. It was tested on Western Blots with the tissue lysates from human, mouse, and rat for specificity.

	Western blot analysis of extracts from LOVO cells treated with serum 20% 30', using FKHRL1 (Phospho-Ser253) Antibody.
	Jurkat cells were starved for 24 hrs. and stimulated with 200nM PMA for 1 hr.
	An illustrated breakdown of the colorimetric Non-Phospho Cell-Based ELISA assay principles.
	A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits.
	The Anti-GAPDH Antibody is a mouse monoclonal antibody used as a positive control in Assay Biotechnology's Cell-Based ELISA kits. It was tested on Western Blots with the tissue lysates from human, mouse, and rat for specificity.

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IHC：(Immunohistochemistry)

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FKHRL1 (Phospho-Ser253) Fluorometric Cell-Based ELISA Kit

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