The FADD (Phospho-Ser191) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect FADD protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated FADD in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on FADD phosphorylation.
Details
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Product Name
FADD (Phospho-Ser191) Colorimetric Cell-Based ELISA Kit
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Catalog No
CBP1858
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Detection Method
Colorimetric 450 nm
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Dynamic Range
> 5000 Cells
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Storage/Stability
4°C/6 Months
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Reactivity
Mouse
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Assay Type
Cell-Based
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Database Links
Gene ID: 14082, UniProt ID: Q61160, OMIM #: None, Unigene #: Mm.5126
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Format
2 x 96-Well Microplates
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Manual (PDF)
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NCBI Gene Symbol
FADD
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Sub Type
Phospho
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Synonyms
FAS-associating death domain-containing protein, Mediator of receptor induced toxicity, MORT1
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TargetName
FADD (Phospho-Ser191)
Application Images
Western blot analysis of extracts from Jurkat cells treated with PMA 125ng/ml 30', using FADD (Phospho-Ser191) Antibody.
An illustrated breakdown of the colorimetric Non-Phospho Cell-Based ELISA assay principles.
A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits.
The Anti-GAPDH Antibody is a mouse monoclonal antibody used as a positive control in Assay Biotechnology's Cell-Based ELISA kits. It was tested on Western Blots with the tissue lysates from human, mouse, and rat for specificity.
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Western blot analysis of extracts from Jurkat cells treated with PMA 125ng/ml 30', using FADD (Phospho-Ser191) Antibody. |
|
An illustrated breakdown of the colorimetric Non-Phospho Cell-Based ELISA assay principles. |
|
A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits. |
|
The Anti-GAPDH Antibody is a mouse monoclonal antibody used as a positive control in Assay Biotechnology's Cell-Based ELISA kits. It was tested on Western Blots with the tissue lysates from human, mouse, and rat for specificity. |
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