Anti-EGFR (Phospho-Thr678) polyclonal Antibody detects EGFR only when phosphorylated at Thr678. Validated for WB, IF and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
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Product Name
EGFR (Phospho-Thr678) Antibody
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Catalog No
A0008
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Storage/Stability
Stable at -20°C for at least 1 year.
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Applications
WB, IF, ELISA
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Clonality
Polyclonal
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Host Species
Rabbit
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Reactivity
Human, Mouse, Rat
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Application Dilutions
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:40000
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Database Links
Gene ID: 1956, Swiss-Prot #: P00533, OMIM #: 131550/211980, Unigene #: Hs.488293
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Immunogen
The antiserum was produced against synthesized peptide derived from human EGFR around the phosphorylation site of Thr678.
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Immunogen Range
651-700
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Modification Sites
H: T678 M: T680 R: T679
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MW
134 kDa
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NCBI Gene Symbol
EGFR
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Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
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Specificity
EGFR (Phospho-Thr678) Antibody detects endogenous levels of EGFR only when phosphorylated at Thr678.
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Synonyms
Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1 , EGFR , ERBB1
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Target Modification
Phospho
Application Images
Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using EGFR (Phospho-Thr678) Antibody. The lane on the right is blocked with the phospho peptide.
Immunofluorescence analysis of HUVEC cells, using EGFR (Phospho-Thr678) Antibody. The picture on the right is blocked with the phospho peptide.
Western Blot analysis of various cells using Phospho-EGFR (T678) Antibody diluted at 1:1000
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Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using EGFR (Phospho-Thr678) Antibody. The lane on the right is blocked with the phospho peptide. |
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Immunofluorescence analysis of HUVEC cells, using EGFR (Phospho-Thr678) Antibody. The picture on the right is blocked with the phospho peptide. |
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Western Blot analysis of various cells using Phospho-EGFR (T678) Antibody diluted at 1:1000 |
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