Anti-EFNB1/2 (Phospho-Tyr330) polyclonal Antibody detects EFNB1/2 only when phosphorylated at Tyr330. Validated for WB and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
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Product Name
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Catalog No
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Storage/Stability
Stable at -20°C for at least 1 year.
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Applications
WB, ELISA
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Clonality
Polyclonal
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Host Species
Rabbit
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Reactivity
Human, Mouse, Rat
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Application Dilutions
WB: 1:500~1:1000 ELISA: 1:40000
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Database Links
Gene ID: 1948, Swiss-Prot #: P52799, OMIM #: 600527, Unigene #: Hs.149239
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Immunogen
The antiserum was produced against synthesized peptide derived from human EFNB1/2 around the phosphorylation site of Tyr330.
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Immunogen Range
284-333
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Modification Sites
H: Y330 M: Y333 R: Y342
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MW
36 kDa
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NCBI Gene Symbol
EFNB2
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Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
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Specificity
EFNB1/2 (Phospho-Tyr330) Antibody detects endogenous levels of EFNB1/2 only when phosphorylated at Tyr330.
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Synonyms
CEK5 ligand, CEK5 receptor ligand, CEK5-L, CEL5-L, EFL-3, EFNB1, ELK ligand, ELK-L, EPH-related receptor tyrosine kinase ligand 2, EPL2, EPLG2, Ephrin-B1 precursor, LERK-2, LERK2, STRA1, STRA1 protein, kinase ephrin-B1
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Target Modification
Phospho
Application Images
| Image 1 |
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Western blot analysis of lysates from 293 cells treated with TNF-a 20ng/ml 30', using EFNB1/2 (Phospho-Tyr330) Antibody. The lane on the left is blocked with the phospho peptide. |
| Image 2 |
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Western Blot analysis of various cells using Phospho-Ephrin-B1/2 (Y330) Antibody |
