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DARPP-32 (Phospho-Thr75) Colorimetric Cell-Based ELISA Kit

Catalog No : CBP1007
  • Reactivity
    Human, Mouse, Rat
  • Size
    1 Kit
  • Price
    $480.00
The DARPP-32 (Phospho-Thr75) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect DARPP-32 protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated DARPP-32 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on DARPP-32 phosphorylation.
Details
  • Product Name
    DARPP-32 (Phospho-Thr75) Colorimetric Cell-Based ELISA Kit
  • Catalog No
    CBP1007
  • Detection Method
    Colorimetric 450 nm
  • Dynamic Range
    > 5000 Cells
  • Storage/Stability
    4°C/6 Months
  • Reactivity
    Human, Mouse, Rat
  • Assay Type
    Cell-Based
  • Database Links
    Gene ID: 84152, UniProt ID: Q9UD71, OMIM #: 604399, Unigene #: Hs.286192
  • Format
    2 x 96-Well Microplates
  • Manual (PDF)
  • NCBI Gene Symbol
    PPP1R1B
  • Sub Type
    Phospho
  • Synonyms
    Dopamine- and cAMP-regulated neuronal phosphoprotein, IPPD, Neuronal phosphoprotein DARPP-32, PPP1R1B
  • TargetName
    DARPP-32 (Phospho-Thr75)
Application Images
Western blot analysis of extracts from COS7 cells treated with Forskolin 40nM 30', using DARPP-32 (Phospho-Thr75) Antibody.
An illustrated breakdown of the colorimetric Non-Phospho Cell-Based ELISA assay principles.
A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits.
The Anti-GAPDH Antibody is a mouse monoclonal antibody used as a positive control in Assay Biotechnology's Cell-Based ELISA kits. It was tested on Western Blots with the tissue lysates from human, mouse, and rat for specificity.
Western blot analysis of extracts from COS7 cells treated with Forskolin 40nM 30', using DARPP-32 (Phospho-Thr75) Antibody.
An illustrated breakdown of the colorimetric Non-Phospho Cell-Based ELISA assay principles.
A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits.
The Anti-GAPDH Antibody is a mouse monoclonal antibody used as a positive control in Assay Biotechnology's Cell-Based ELISA kits. It was tested on Western Blots with the tissue lysates from human, mouse, and rat for specificity.