The DARPP-32 (Phospho-Thr75) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect DARPP-32 protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated DARPP-32 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on DARPP-32 phosphorylation.
Details
-
Product Name
DARPP-32 (Phospho-Thr75) Colorimetric Cell-Based ELISA Kit
-
Catalog No
CBP1007
-
Detection Method
Colorimetric 450 nm
-
Dynamic Range
> 5000 Cells
-
Storage/Stability
4°C/6 Months
-
Reactivity
Human, Mouse, Rat
-
Assay Type
Cell-Based
-
Database Links
Gene ID: 84152, UniProt ID: Q9UD71, OMIM #: 604399, Unigene #: Hs.286192
-
Format
2 x 96-Well Microplates
-
Manual (PDF)
-
NCBI Gene Symbol
PPP1R1B
-
Sub Type
Phospho
-
Synonyms
Dopamine- and cAMP-regulated neuronal phosphoprotein, IPPD, Neuronal phosphoprotein DARPP-32, PPP1R1B
-
TargetName
DARPP-32 (Phospho-Thr75)
Application Images
Western blot analysis of extracts from COS7 cells treated with Forskolin 40nM 30', using DARPP-32 (Phospho-Thr75) Antibody.
An illustrated breakdown of the colorimetric Non-Phospho Cell-Based ELISA assay principles.
A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits.
The Anti-GAPDH Antibody is a mouse monoclonal antibody used as a positive control in Assay Biotechnology's Cell-Based ELISA kits. It was tested on Western Blots with the tissue lysates from human, mouse, and rat for specificity.
|
Western blot analysis of extracts from COS7 cells treated with Forskolin 40nM 30', using DARPP-32 (Phospho-Thr75) Antibody. |
|
An illustrated breakdown of the colorimetric Non-Phospho Cell-Based ELISA assay principles. |
|
A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits. |
|
The Anti-GAPDH Antibody is a mouse monoclonal antibody used as a positive control in Assay Biotechnology's Cell-Based ELISA kits. It was tested on Western Blots with the tissue lysates from human, mouse, and rat for specificity. |
Follow us on Twitter
Follow us on Linkedin
Like us on Facebook
Subscribe to our Channel