Anti-DARPP-32 (Phospho-Thr75) polyclonal Antibody detects DARPP-32 only when phosphorylated at Thr75. Validated for WB, IHC and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
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Product Name
DARPP-32 (Phospho-Thr75) Antibody
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Catalog No
A0007
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Storage/Stability
Stable at -20°C for at least 1 year.
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Applications
WB, IHC, ELISA
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Clonality
Polyclonal
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Host Species
Rabbit
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Reactivity
Human, Mouse, Rat
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Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000
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Database Links
Gene ID: 84152, Swiss-Prot #: Q9UD71, OMIM #: 604399, Unigene #: Hs.286192
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Immunogen
The antiserum was produced against synthesized peptide derived from human DARPP-32 around the phosphorylation site of Thr75.
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Immunogen Range
41-90
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Modification Sites
H: T75 M: T75 R: T75
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MW
22 kDa
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NCBI Gene Symbol
PPP1R1B
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Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
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Specificity
DARPP-32 (Phospho-Thr75) Antibody detects endogenous levels of DARPP-32 only when phosphorylated at Thr75.
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Synonyms
Dopamine- and cAMP-regulated neuronal phosphoprotein, IPPD, Neuronal phosphoprotein DARPP-32, PPP1R1B
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Target Modification
Phospho
Application Images
Western blot analysis of lysates from COS7 cells treated with Forskolin 40nM 30', using DARPP-32 (Phospho-Thr75) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using DARPP-32 (Phospho-Thr75) Antibody. The picture on the right is blocked with the phospho peptide.
Western Blot analysis of various cells using Phospho-DARPP-32 (T75) Antibody diluted at 1:500
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4°,ON). Negative control (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,ON). Negative control (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Western blot analysis of lysates from COS7 cells treated with Forskolin 40nM 30', using DARPP-32 (Phospho-Thr75) Antibody. The lane on the right is blocked with the phospho peptide. |
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using DARPP-32 (Phospho-Thr75) Antibody. The picture on the right is blocked with the phospho peptide. |
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Western Blot analysis of various cells using Phospho-DARPP-32 (T75) Antibody diluted at 1:500 |
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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4°,ON). Negative control (right) obtaned from antibody was pre-absorbed by immunogen peptide. |
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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,ON). Negative control (right) obtaned from antibody was pre-absorbed by immunogen peptide. |
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