Anti-DARPP-32 (Phospho-Thr75) Antibody (A0007) Rabbit Polyclonal

Description

Anti-DARPP-32 (Phospho-Thr75) polyclonal Antibody detects DARPP-32 only when phosphorylated at Thr75. Validated for WB, IHC and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.

Details

Product Name

DARPP-32 (Phospho-Thr75) Antibody
Catalog No

A0007
Storage/Stability

Stable at -20°C for at least 1 year.
Applications

WB, IHC, ELISA
Clonality

Polyclonal
Host Species

Rabbit
Reactivity

Human, Mouse, Rat
Application Dilutions

WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000
Database Links

Gene ID: 84152, Swiss-Prot #: Q9UD71, OMIM #: 604399, Unigene #: Hs.286192
Immunogen

The antiserum was produced against synthesized peptide derived from human DARPP-32 around the phosphorylation site of Thr75.
Immunogen Range

41-90
Modification Sites

H: T75 M: T75 R: T75
MW

22 kDa
NCBI Gene Symbol

PPP1R1B
Physical Form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification

The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Specificity

DARPP-32 (Phospho-Thr75) Antibody detects endogenous levels of DARPP-32 only when phosphorylated at Thr75.
Synonyms

Dopamine- and cAMP-regulated neuronal phosphoprotein, IPPD, Neuronal phosphoprotein DARPP-32, PPP1R1B
Target Modification

Phospho

Application Images

Image 1		Western blot analysis of lysates from COS7 cells treated with Forskolin 40nM 30', using DARPP-32 (Phospho-Thr75) Antibody. The lane on the right is blocked with the phospho peptide.
Image 2		Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using DARPP-32 (Phospho-Thr75) Antibody. The picture on the right is blocked with the phospho peptide.
Image 3		Western Blot analysis of various cells using Phospho-DARPP-32 (T75) Antibody diluted at 1:500
Image 4		Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4°,ON). Negative control (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Image 5		Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,ON). Negative control (right) obtaned from antibody was pre-absorbed by immunogen peptide.

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By Application

IHC：(Immunohistochemistry)

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