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CrkII (Phospho-Tyr221) Fluorometric Cell-Based ELISA Kit

Catalog No : FLUO-CBP1059
  • Reactivity
    Human , Mouse , Rat
  • Assay Type
    Cell-Based ELISA
  • Size
    1 Kit
  • Price
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The CrkII (Phospho-Tyr221) Fluorometric Cell-Based Phospho ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor CrkII (Phospho-Tyr221) protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated CrkII (Phospho-Tyr221) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals, or activators have on CRK phosphorylation.
  • Product Name
  • Catalog No
  • Detection Method
  • Dynamic Range
    > 5000 Cells
  • Storage/Stability
    4°C/6 Months
  • Reactivity
    Human, Mouse, Rat
  • Assay Type
    Cell-Based ELISA
  • Database Links
    Gene ID: 1398, UniProt ID: P46108, OMIM #: 164762, Unigene #: Hs.638121
  • Format
    Two 96-Well Plates
  • Manual (PDF)
  • NCBI Gene Symbol
  • Sub Type
  • Synonyms
    Adapter molecule crk, CRKO, Crk-II, P38, Proto-oncogene C-crk
  • TargetName
    Phospho-CrkII (Tyr221)
Application Images
Image 1 Western blot analysis of extracts from COS7 cells, using CrkII (Phospho-Tyr221) Antibody.
Image 2 HeLa cells were starved for 24 hrs. and stimulated with Etoposide 25uM for 1 hr.
Image 3 An illustrated breakdown of the colorimetric Non-Phospho Cell-Based ELISA assay principles.
Image 4 A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits.
Image 5 The Anti-GAPDH Antibody is a mouse monoclonal antibody used as a positive control in Assay Biotechnology's Cell-Based ELISA kits. It was tested on Western Blots with the tissue lysates from human, mouse, and rat for specificity.
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