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Caspase 6 (Phospho-Ser257) Antibody

Catalog No : A0058
  • Applications
    WB, IHC, IF, ELISA
  • Reactivity
    Human, Mouse, Rat
  • Size
    50ul
  • Price
    $195.00
Anti-Caspase 6 (Phospho-Ser257) polyclonal Antibody detects Caspase 6 only when phosphorylated at Ser257. Validated for WB, IHC, IF and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
  • Product Name
    Caspase 6 (Phospho-Ser257) Antibody
  • Catalog No
    A0058
  • Storage/Stability
    Stable at -20°C for at least 1 year.
  • Applications
    WB, IHC, IF, ELISA
  • Clonality
    Polyclonal
  • Host Species
    Rabbit
  • Reactivity
    Human, Mouse, Rat
  • Application Dilutions
    WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000
  • Database Links
    Gene ID: 839, Swiss-Prot #: P55212, OMIM #: 601532, Unigene #: Hs.654616
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human Caspase 6 around the phosphorylation site of Ser257.
  • Immunogen Range
    223-272
  • Modification Sites
    H: S257 M: S239 R: S240
  • MW
    33 kDa
  • NCBI Gene Symbol
    CASP6
  • Physical Form
    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Purification
    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
  • Specificity
    Caspase 6 (Phospho-Ser257) Antibody detects endogenous levels of Caspase 6 only when phosphorylated at Ser257.
  • Synonyms
    ICE6, MCH2, apoptotic protease Mch-2, caspase-6
  • Target Modification
    Phospho
Application Images
Western blot analysis of lysates from 293 cells treated with Etoposide 25uM 60', using Caspase 6 (Phospho-Ser257) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using Caspase 6 (Phospho-Ser257) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of HUVEC cells, using Caspase 6 (Phospho-Ser257) Antibody. The picture on the right is blocked with the phospho peptide.
Western Blot analysis of 293T cells using Phospho-Caspase-6 (S257) Antibody diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4°,ON). Negative control (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Western blot analysis of lysates from 293 cells treated with Etoposide 25uM 60', using Caspase 6 (Phospho-Ser257) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using Caspase 6 (Phospho-Ser257) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of HUVEC cells, using Caspase 6 (Phospho-Ser257) Antibody. The picture on the right is blocked with the phospho peptide.
Western Blot analysis of 293T cells using Phospho-Caspase-6 (S257) Antibody diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4°,ON). Negative control (right) obtaned from antibody was pre-absorbed by immunogen peptide.