(408) 747-0185

Calnexin (Phospho-Ser583) Antibody

Catalog No : A0463
  • Applications
    WB, IHC, IF, ELISA
  • Reactivity
    Human, Mouse, Rat
  • Size
    50ul
  • Price
    $195.00
Anti-Calnexin (Phospho-Ser583) polyclonal Antibody detects Calnexin only when phosphorylated at Ser583. Validated for WB, IHC, IF and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
  • Product Name
    Calnexin (Phospho-Ser583) Antibody
  • Catalog No
    A0463
  • Storage/Stability
    Stable at -20°C for at least 1 year.
  • Applications
    WB, IHC, IF, ELISA
  • Clonality
    Polyclonal
  • Host Species
    Rabbit
  • Reactivity
    Human, Mouse, Rat
  • Application Dilutions
    WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000
  • Database Links
    Gene ID: 821, Swiss-Prot #: P27824, OMIM #: 114217, Unigene #: Hs.699155
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human Calnexin around the phosphorylation site of Ser583.
  • Immunogen Range
    543-592
  • Modification Sites
    H: S583 M: S582 R: S582
  • MW
    67 kDa
  • NCBI Gene Symbol
    CANX
  • Physical Form
    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Purification
    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
  • Specificity
    Calnexin (Phospho-Ser583) Antibody detects endogenous levels of Calnexin only when phosphorylated at Ser583.
  • Synonyms
    CALX, CANX, IP90, Major histocompatibility complex class I
  • Target Modification
    Phospho
Application Images
Western blot analysis of lysates from HeLa cells treated with EGF 200ng/ml 30', using Calnexin (Phospho-Ser583) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Calnexin (Phospho-Ser583) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of HeLa cells, using Calnexin (Phospho-Ser583) Antibody. The picture on the right is blocked with the phospho peptide.
Western Blot analysis of various cells using Phospho-Calnexin (S583) Antibody diluted at 1:2000
Western blot analysis of lysates from HeLa cells treated with EGF 200ng/ml 30', using Calnexin (Phospho-Ser583) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Calnexin (Phospho-Ser583) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of HeLa cells, using Calnexin (Phospho-Ser583) Antibody. The picture on the right is blocked with the phospho peptide.
Western Blot analysis of various cells using Phospho-Calnexin (S583) Antibody diluted at 1:2000