Anti-Calnexin Antibody detects endogenous levels of total Calnexin protein. Validated for WB, IHC, IF, and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
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Product Name
Calnexin Antibody
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Catalog No
B0463
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Storage/Stability
Stable at -20°C for at least 1 year.
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Applications
WB, IHC, IF, ELISA
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Clonality
Polyclonal
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Host Species
Rabbit
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Reactivity
Human, Mouse, Rat
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Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000
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Database Links
Gene ID: 821, Swiss-Prot #: P27824, OMIM #: 114217, Unigene #: Hs.567968
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Immunogen
The antiserum was produced against synthesized peptide derived from human Calnexin.
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Immunogen Range
543-592
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MW
67 kDa
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NCBI Gene Symbol
CANX
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Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.
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Specificity
Calnexin Antibody detects endogenous levels of total Calnexin protein.
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Synonyms
CALX, CANX, IP90, Major histocompatibility complex class I
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Target Modification
Unmodified/Total
Application Images
Western blot analysis of lysates from HeLa cells, treated with EGF 200ng/ml 30', using Calnexin Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Calnexin Antibody. The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, using Calnexin Antibody. The picture on the right is blocked with the synthesized peptide.
Western Blot analysis of various cells using primary antibody diluted at 1:1000(4°C ON). Secondary antibody?Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour).
Western Blot analysis of various cells using Calnexin Antibody diluted at 1:2000
Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. Calnexin Antibody was diluted at 1:200(4°C,ON). Negative control was defined by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. Calnexin Antibody was diluted at 1:200(4°C,ON). Negative control was defined by secondary antibody only.
Immunofluorescence analysis of rat-spleen tissue. Calnexin Antibody(red) was diluted at 1:200(4°C,ON). Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of rat-lung tissue. Calnexin Antibody(red) was diluted at 1:200(4°C,ON). Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
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Western blot analysis of lysates from HeLa cells, treated with EGF 200ng/ml 30', using Calnexin Antibody. The lane on the right is blocked with the synthesized peptide. |
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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Calnexin Antibody. The picture on the right is blocked with the synthesized peptide. |
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Immunofluorescence analysis of HeLa cells, using Calnexin Antibody. The picture on the right is blocked with the synthesized peptide. |
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Western Blot analysis of various cells using primary antibody diluted at 1:1000(4°C ON). Secondary antibody?Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour). |
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Western Blot analysis of various cells using Calnexin Antibody diluted at 1:2000 |
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Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. Calnexin Antibody was diluted at 1:200(4°C,ON). Negative control was defined by secondary antibody only. |
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Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. Calnexin Antibody was diluted at 1:200(4°C,ON). Negative control was defined by secondary antibody only. |
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Immunofluorescence analysis of rat-spleen tissue. Calnexin Antibody(red) was diluted at 1:200(4°C,ON). Picture A:Target. Picture B: DAPI. Picture C: merge of A+B |
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Immunofluorescence analysis of rat-lung tissue. Calnexin Antibody(red) was diluted at 1:200(4°C,ON). Picture A:Target. Picture B: DAPI. Picture C: merge of A+B |