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Calnexin Antibody

Catalog No : B0463
  • Applications
    WB, IHC, IF, ELISA
  • Reactivity
    Human, Mouse, Rat
  • Size
    50ul
  • Price
    $195.00
Anti-Calnexin Antibody detects endogenous levels of total Calnexin protein. Validated for WB, IHC, IF, and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
  • Product Name
    Calnexin Antibody
  • Catalog No
    B0463
  • Storage/Stability
    Stable at -20°C for at least 1 year.
  • Applications
    WB, IHC, IF, ELISA
  • Clonality
    Polyclonal
  • Host Species
    Rabbit
  • Reactivity
    Human, Mouse, Rat
  • Application Dilutions
    WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000
  • Database Links
    Gene ID: 821, Swiss-Prot #: P27824, OMIM #: 114217, Unigene #: Hs.567968
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human Calnexin.
  • Immunogen Range
    543-592
  • MW
    67 kDa
  • NCBI Gene Symbol
    CANX
  • Physical Form
    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Purification
    The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.
  • Specificity
    Calnexin Antibody detects endogenous levels of total Calnexin protein.
  • Synonyms
    CALX, CANX, IP90, Major histocompatibility complex class I
  • Target Modification
    Unmodified/Total
Application Images
Western blot analysis of lysates from HeLa cells, treated with EGF 200ng/ml 30', using Calnexin Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Calnexin Antibody. The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, using Calnexin Antibody. The picture on the right is blocked with the synthesized peptide.
Western Blot analysis of various cells using primary antibody diluted at 1:1000(4°C ON). Secondary antibody?Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour).
Western Blot analysis of various cells using Calnexin Antibody diluted at 1:2000
Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. Calnexin Antibody was diluted at 1:200(4°C,ON). Negative control was defined by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. Calnexin Antibody was diluted at 1:200(4°C,ON). Negative control was defined by secondary antibody only.
Immunofluorescence analysis of rat-spleen tissue. Calnexin Antibody(red) was diluted at 1:200(4°C,ON). Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of rat-lung tissue. Calnexin Antibody(red) was diluted at 1:200(4°C,ON). Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Western blot analysis of lysates from HeLa cells, treated with EGF 200ng/ml 30', using Calnexin Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Calnexin Antibody. The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, using Calnexin Antibody. The picture on the right is blocked with the synthesized peptide.
Western Blot analysis of various cells using primary antibody diluted at 1:1000(4°C ON). Secondary antibody?Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour).
Western Blot analysis of various cells using Calnexin Antibody diluted at 1:2000
Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. Calnexin Antibody was diluted at 1:200(4°C,ON). Negative control was defined by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. Calnexin Antibody was diluted at 1:200(4°C,ON). Negative control was defined by secondary antibody only.
Immunofluorescence analysis of rat-spleen tissue. Calnexin Antibody(red) was diluted at 1:200(4°C,ON). Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of rat-lung tissue. Calnexin Antibody(red) was diluted at 1:200(4°C,ON). Picture A:Target. Picture B: DAPI. Picture C: merge of A+B