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Calcium Assay Kit II - Fluorometric

Catalog No : EZ-0540
  • Size
    100 Assays
  • Price
This homogeneous fluorescence-based assay pre-loads cells expressing a GPCR of interest that signals through calcium with Fura-2 AM, which can cross cell membrane. Esterases within the cell cleave the lipophilic blocking groups on the Fura-2 AM, producing a fluorescent dye that stays inside the cell. Upon binding to calcium, the fluorescence of this negatively charged product will increase with increasing levels of calcium. This allows us to monitor GPCRs and calcium channels indirectly with a 340/380 nm excitation ratio with no wash step, which reduces the effects of uneven dye loading and cell numbers, dye leakage, and photo bleaching.
  • Product Name
    Calcium Assay Kit II - Fluorometric
  • Catalog No
  • Detection Method
  • Storage/Stability
    -20°, C/3 Months
  • Fluorometric Readout [Ex (nm), Em (nm)]
    340, 510
  • Manual
  • Range Detection
    0.01 mM - 10 mM
  • Tar Name
Application Images
Image 1 The Calcium Assay Kit II - Fluorometric allows for the detection and quantification of endogenous Calcium within the range of 0.01 to 10 µM in cell lysates, sera, and plasma.
Image 2