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CREB (Phospho-Ser133) Antibody

Catalog No : A7053
  • Applications
    WB, IHC, IP, ELISA
  • Reactivity
    Human, Mouse, Rat
  • Size
    50ul
  • Price
    $195.00
Anti-CREB (Phospho-Ser133) polyclonal Antibody detects CREB only when phosphorylated at Ser133. Validated for WB IHC IP ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
  • Product Name
    CREB (Phospho-Ser133) Antibody
  • Catalog No
    A7053
  • Storage/Stability
    Stable at -20°C for at least 1 year.
  • Applications
    WB, IHC, IP, ELISA
  • Clonality
    Polyclonal
  • Host Species
    Rabbit
  • Reactivity
    Human, Mouse, Rat
  • Application Dilutions
    WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
  • Database Links
    Gene ID: 1385, Swiss-Prot #: P16220, OMIM #: 123810/612160, Unigene #: Hs.516646/Hs.584750
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human CREB around the phosphorylation site of Ser133.
  • Immunogen Range
    100-149
  • Modification Sites
    H: S133 M: S133 R: S133
  • MW
    36 kDa
  • NCBI Gene Symbol
    CREB1
  • Physical Form
    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Purification
    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
  • Specificity
    CREB (Phospho-Ser133) Antibody detects endogenous levels of CREB only when phosphorylated at Ser133.
  • Synonyms
    cAMP responsive element binding protein 1, cAMP-response element binding protein, CREB-1, CREB1
  • Target Modification
    Phospho
Application Images
Image 1 Western blot analysis of lysates from Jurkat and 293 cells treated with UV, using CREB (Phospho-Ser133) Antibody. The lane on the right is blocked with the phospho peptide.
Image 2 Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using CREB (Phospho-Ser133) Antibody. The picture on the right is blocked with the phospho peptide.
Image 3 Western Blot analysis of various cells using primary antibody diluted at 1:1000(4°C ON). Secondary antibody?Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour). Western Blot analysis of various cells using Phospho-CREB-1 (S133) Antibody diluted at 1:2000
Image 4 Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. CREB-1 (phospho Ser133) Antibody was diluted at 1:200(4°C,ON). Negative control was defined by secondary antibody only.
Image 5 Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. CREB-1 (phospho Ser133) Antibody was diluted at 1:200(4°C,ON). Negative control was defined by secondary antibody only.
Image 6 Immunofluorescence analysis of mouse-lung tissue. CREB-1 (phospho Ser133) Antibody(red) was diluted at 1:200(4°C,ON). Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Image 7 Immunofluorescence analysis of mouse-testis tissue. CREB-1 (phospho Ser133) Antibody(red) was diluted at 1:200(4°C,ON). Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Image 8